A mild method for fluorescein labeling of base-sensitive oligonucleotides on solid support

被引：6

作者：

Bologna, JC ^{[1
]}

Imbach, JL ^{[1
]}

Morvan, F ^{[1
]}

机构：

[1] Univ Montpellier 2, Lab Chim Organ Biomol Synth, CNRS, UMR 5625,UM 2, F-34095 Montpellier 05, France

来源：

TETRAHEDRON LETTERS | 2000年 / 41卷 / 38期

基金：

澳大利亚研究理事会;

关键词：

fluorescein; base-sensitive oligonucleotides;

D O I：

10.1016/S0040-4039(00)01227-2

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

A simple and mild method is described to covalently label base-sensitive supported oligonucleotides, such as prooligonucleotides, with unprotected carboxyfluorescein as a highly reactive dimethylaminopyridinium ester generated in situ. (C) 2000 Elsevier Science Ltd. All rights reserved.

引用

页码：7317 / 7321

页数：5

共 17 条

[1]

Bologna JC, 1999, EUR J ORG CHEM, V1999, P2353

[2] Photolabile linker for the solid-phase synthesis of base-sensitive oligonucleotides [J].

DellAquila, C ;

Imbach, JL ;

Rayner, B .

TETRAHEDRON LETTERS, 1997, 38 (30) :5289-5292

[3] Polyethylenimine but not cationic lipid improves antisense activity of 3'-capped phosphodiester oligonucleolides [J].

Dheur, S ;

Dias, N ;

Van Aerschot, A ;

Herdewijn, P ;

Bettinger, T ;

Rémy, JS ;

Hélène, C ;

Saison-Behmoaras, ET .

ANTISENSE & NUCLEIC ACID DRUG DEVELOPMENT, 1999, 9 (06) :515-525

[4] Sequence verification by hybridisation with fluorescent octanucleotides as a first step to a fluorescent sequencing by hybridisation protocol [J].

Eickhoff, H ;

BirchHirschfeld, E ;

Scheef, J ;

Hoyer, C ;

Drexhage, KH ;

Greulich, KO .

JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS, 1996, 32 (01) :59-68

[5]

Lakowicz J. R., 2010, PRINCIPLES FLUORESCE

[6] A new method of synthesis of fluorescently labelled oligonucleotides and their application in DNA sequencing [J].

Markiewicz, WT ;

Groger, G ;

Rosch, R ;

Zebrowska, A ;

Markiewicz, M ;

Klotz, M ;

Hinz, M ;

Godzina, P ;

Seliger, H .

NUCLEIC ACIDS RESEARCH, 1997, 25 (18) :3672-3680

[7] A CONVENIENT AND GENERAL-APPROACH TO THE SYNTHESIS OF PROPERLY PROTECTED D-NUCLEOSIDE-3'-HYDROGENPHOSPHONATES VIA PHOSPHITE INTERMEDIATES [J].

MARUGG, JE ;

TROMP, M ;

KUYLYEHESKIELY, E ;

VANDERMAREL, GA ;

VANBOOM, JH .

TETRAHEDRON LETTERS, 1986, 27 (23) :2661-2664

[8] FLUORESCENCE ENERGY-TRANSFER AS A PROBE FOR NUCLEIC-ACID STRUCTURES AND SEQUENCES [J].

MERGNY, JL ;

BOUTORINE, AS ;

GARESTIER, T ;

BELLOC, F ;

ROUGEE, M ;

BULYCHEV, NV ;

KOSHKIN, AA ;

BOURSON, J ;

LEBEDEV, AV ;

VALEUR, B ;

THUONG, NT ;

HELENE, C .

NUCLEIC ACIDS RESEARCH, 1994, 22 (06) :920-928

[9]

MORVAN F, 2000, T&F SER PHARM SCI, P79

[10] Fluorescent-labeled oligonucleotide probes with non-nucleotide linker: Detection of hybrid formation by fluorescence anisotropy [J].

Patil, SV ;

Salunkhe, MM .

NUCLEOSIDES & NUCLEOTIDES, 1996, 15 (10) :1603-1610

← 1 2 →