Test compounds for detecting the silanol effect on the elution of ionized amines in reversed-phase LC

被引：25

作者：

Okusa, Kensuke ^{[2
]}

Suita, Yuki ^{[2
]}

Otsuka, Yukio ^{[3
]}

Tahara, Mineo ^{[3
]}

Ikegami, Tohru ^{[1
]}

Tanaka, Nobuo ^{[1
,2
]}

Ohira, Masayoshi ^{[2
]}

Takahashi, Masakazu ^{[2
]}

机构：

[1] Kyoto Inst Technol, Sakyo Ku, Kyoto 6068585, Japan

[2] GL Sci Inc, Iruma, Saitama, Japan

[3] GL Sci Inc, Fukushima, Japan

来源：

JOURNAL OF SEPARATION SCIENCE | 2010年 / 33卷 / 03期

关键词：

Basic compound; Reversed-phase; Silanol effect; PERFORMANCE LIQUID-CHROMATOGRAPHY; STRONGLY BASIC COMPOUNDS; COLUMN SELECTIVITY; DEXTROMETHORPHAN HYDROBROMIDE; CHLORPHENIRAMINE MALEATE; UNIVERSAL PROCEDURE; TEST PARAMETERS; MINIMAL NUMBER; CLASSIFICATION; RETENTION;

D O I：

10.1002/jssc.200900760

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The effectiveness of several basic compounds for testing silica-based stationary phases was reviewed by applying them to recent columns for reversed-phase H PLC Most octadecylsilylated (C18) stationary phases, prepared as a base-deactivated material from high-purity silica gel with endcapping, provided excellent peak shape and column efficiency for the bases including benzylamine and amitriptyline that once caused problems and were subsequently employed for testing silanol activities However, a cyclic tertiary amine, dextrometorphan, was eluted as an acceptable peak from only a few columns at neutral pH. Such a more sensitive probe is expected to contribute to further improvement of the stationary phase for reversed-phase HPLC

引用

页码：348 / 358

页数：11

共 63 条

[1] Determination of paracetamol:: Historical evolution [J].