Detection and quantification of macrolide resistance mutations at positions 2058 and 2059 of the 23S rRNA gene by pyrosequencing

被引：47

作者：

Haanperä, M

Huovinen, P

Jalava, J

机构：

[1] Natl Publ Hlth Inst, Human Microbial Ecol Lab, FI-20520 Turku, Finland

[2] Natl Publ Hlth Inst, Antimicrobial Res Lab, FI-20520 Turku, Finland

来源：

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY | 2005年 / 49卷 / 01期

关键词：

D O I：

10.1128/AAC.49.1.457-460.2005

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A pyrosequencing method for detection and quantification of macrolide resistance mutations at positions 2058 and 2059 (Escherichia coli numbering) of the 23S rRNA gene is described. The method was developed and tested for Streptococcus pneumoniae, Streptococcus pyogenes, Mycobacterium avium, Campylobacter jejuni, and Haemophilus influenzae.

引用

收藏

页码：457 / 460

页数：4

相关论文

共 14 条

[1] Denaturing high-performance liquid chromatography detection of ribosomal mutations conferring macrolide resistance in gram-positive cocci [J].

Canu, A ;

Abbas, A ;

Malbruny, B ;

Sichel, F ;

Leclercq, R .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2004, 48 (01) :297-304

[2] Mutation at the position 2058 of the 23S rRNA as a cause of macrolide resistance in Streptococcus pyogenes [J].

Jalava J. ;

Vaara M. ;

Huovinen P. .

Annals of Clinical Microbiology and Antimicrobials, 3 (1)

[3] Fast and accurate quantitative detection of Helicobacter pylori and identification of clarithromycin resistance mutations in H-pylori isolates from gastric biopsy specimens by real-time PCR [J].

Lascols, C ;

Lamarque, D ;

Costa, JM ;

Copie-Bergman, C ;

Le Glaunec, JM ;

Deforges, L ;

Soussy, CJ ;

Petit, JC ;

Delchier, JC ;

Tankovic, J .

JOURNAL OF CLINICAL MICROBIOLOGY, 2003, 41 (10) :4573-4577

[4] IDENTIFICATION OF MUTATIONS IN 23S RIBOSOMAL-RNA GENE OF CLARITHROMYCIN-RESISTANT MYCOBACTERIUM-INTRACELLULARE [J].

MEIER, A ;

KIRSCHNER, P ;

SPRINGER, B ;

STEINGRUBE, VA ;

BROWN, BA ;

WALLACE, RJ ;

BOTTGER, EC .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1994, 38 (02) :381-384

[5] Rapid molecular identification and subtyping of Helicobacter pylori by pyrosequencing of the 16S rDNA variable V1 and V3 regions [J].

Monstein, HJ ;

Nikpour-Badr, S ;

Jonasson, J .

FEMS MICROBIOLOGY LETTERS, 2001, 199 (01) :103-107

[6] GENETIC-BASIS OF MACROLIDE RESISTANCE IN MYCOBACTERIUM-AVIUM ISOLATED FROM PATIENTS WITH DISSEMINATED DISEASE [J].

NASH, KA ;

INDERLIED, CB .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1995, 39 (12) :2625-2630

[7] Monitoring resistance to human immunodeficiency virus type 1 protease inhibitors by pyrosequencing [J].

O'Meara, D ;

Wilbe, K ;

Leitner, T ;

Hejdeman, B ;

Albert, J ;

Lundeberg, J .

JOURNAL OF CLINICAL MICROBIOLOGY, 2001, 39 (02) :464-473

[8] Ribosomal mutations in Streptococcus pneumoniae clinical isolates [J].

Pihlajamäki, M ;

Kataja, J ;

Seppälä, H ;

Elliot, J ;

Leinonen, M ;

Huovinen, P ;

Jalava, J .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2002, 46 (03) :654-658

[9] Clinical isolates of Staphylococcus aureus with ribosomal mutations conferring resistance to macrolides [J].

Prunier, AL ;

Malbruny, B ;

Tandé, D ;

Picard, B ;

Leclercq, R .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2002, 46 (09) :3054-3056

[10] A sequencing method based on real-time pyrophosphate [J].

Ronaghi, M ;

Uhlén, M ;

Nyrén, P .

SCIENCE, 1998, 281 (5375) :363-+