Hybrid bilayer membranes in air and water: Infrared spectroscopy and neutron reflectivity studies

被引:110
作者
Meuse, CW
Krueger, S
Majkrzak, CF
Dura, JA
Fu, J
Connor, JT
Plant, AL
机构
[1] NIST, Div Biotechnol, Gaithersburg, MD 20899 USA
[2] NIST, Ctr Neutron Res, Gaithersburg, MD 20899 USA
[3] NIST, Div Precis Engn, Gaithersburg, MD 20899 USA
关键词
D O I
10.1016/S0006-3495(98)77851-8
中图分类号
Q6 [生物物理学];
学科分类号
071011 ;
摘要
In this report we describe the fabrication and characterization of a phospholipid/alkanethiol hybrid bilayer membrane in air. The bilayer is formed by the interaction of phospholipid with the hydrophobic surface of a self-assembled alkanethiol monolayer on gold. We have characterized the resulting hybrid bilayer membrane in air using atomic force microscopy, spectroscopic ellipsometry, and reflection-absorption infrared spectroscopy. These analyses indicate that the phospholipid added is one monolayer thick, is continuous, and exhibits molecular order which is similar to that observed for phospholipid/phospholipid model membranes. The hybrid bilayer prepared in air has also been re-introduced to water and characterized using neutron reflectivity and impedance spectroscopy. Impedance data indicate that when moved from air to water, hybrid bilayers exhibit a dielectric constant and thickness that is essentially equivalent to hybrid bilayers prepared in situ by adding phospholipid vesicles to alkanethiol monolayers in water. Neutron scattering from these samples was collected out to a wave vector transfer of 0.25 Angstrom(-1), and provided a sensitivity to changes in total layer thickness on the order of 1-2 Angstrom. The data confirm that the acyl chain region of the phospholipid layer is consistent with that observed for phospholipid-phospholipid bilayers, but suggest greater hydration of the phospholipid headgroups of HBMs than has been reported in studies of lipid multilayers.
引用
收藏
页码:1388 / 1398
页数:11
相关论文
共 44 条
[21]   STRUCTURE OF FULLY HYDRATED BILAYER DISPERSIONS [J].
NAGLE, JF ;
WIENER, MC .
BIOCHIMICA ET BIOPHYSICA ACTA, 1988, 942 (01) :1-10
[22]   LECITHIN BILAYERS - DENSITY-MEASUREMENTS AND MOLECULAR-INTERACTIONS [J].
NAGLE, JF ;
WILKINSON, DA .
BIOPHYSICAL JOURNAL, 1978, 23 (02) :159-175
[23]   X-ray structure determination of fully hydrated L(alpha) phase dipalmitoylphosphatidylcholine bilayers [J].
Nagle, JF ;
Zhang, RT ;
TristramNagle, S ;
Sun, WJ ;
Petrache, HI ;
Suter, RM .
BIOPHYSICAL JOURNAL, 1996, 70 (03) :1419-1431
[24]   STRUCTURE OF MIXED MONOLAYERS OF DIPALMITOYLGLYCEROPHOSPHOCHOLINE AND POLYETHYLENE-GLYCOL MONODODECYL ETHER AT THE AIR-WATER-INTERFACE DETERMINED BY NEUTRON REFLECTION AND FILM BALANCE TECHNIQUES [J].
NAUMANN, C ;
DIETRICH, C ;
LU, JR ;
THOMAS, RK ;
RENNIE, AR ;
PENFOLD, J ;
BAYERL, TM .
LANGMUIR, 1994, 10 (06) :1919-1925
[25]   STUDIES OF THE TEMPERATURE-DEPENDENT PHASE-BEHAVIOR OF LONG-CHAIN NORMAL-ALKYL THIOL MONOLAYERS ON GOLD [J].
NUZZO, RG ;
KORENIC, EM ;
DUBOIS, LH .
JOURNAL OF CHEMICAL PHYSICS, 1990, 93 (01) :767-773
[26]   QUANTITATIVE-DETERMINATION OF MOLECULAR-STRUCTURE IN MULTILAYERED THIN-FILMS OF BIAXIAL AND LOWER SYMMETRY FROM PHOTON SPECTROSCOPIES .1. REFLECTION INFRARED VIBRATIONAL SPECTROSCOPY [J].
PARIKH, AN ;
ALLARA, DL .
JOURNAL OF CHEMICAL PHYSICS, 1992, 96 (02) :927-945
[27]   CORRELATION OF MOLECULAR-ORGANIZATION AND SUBSTRATE WETTABILITY IN THE SELF-ASSEMBLY OF N-ALKYLSILOXANE MONOLAYERS [J].
PARIKH, AN ;
LIEDBERG, B ;
ATRE, SV ;
HO, M ;
ALLARA, DL .
JOURNAL OF PHYSICAL CHEMISTRY, 1995, 99 (24) :9996-10008
[28]  
PEDULLA J, 1993, P SOC PHOTO-OPT INS, V2011, P299
[29]   PHOSPHOLIPID ALKANETHIOL BILAYERS FOR CELL-SURFACE RECEPTOR STUDIES BY SURFACE-PLASMON RESONANCE [J].
PLANT, AL ;
BRIGHAMBURKE, M ;
PETRELLA, EC ;
OSHANNESSY, DJ .
ANALYTICAL BIOCHEMISTRY, 1995, 226 (02) :342-348
[30]   SELF-ASSEMBLED PHOSPHOLIPID ALKANETHIOL BIOMIMETIC BILAYERS ON GOLD [J].
PLANT, AL .
LANGMUIR, 1993, 9 (11) :2764-2767