Lifelong Physical Activity Modulation of the Skeletal Muscle Mitochondrial Proteome in Mice

被引：29

作者：

Alves, Renato M. P. ^{[1
]}

Vitorino, Rui ^{[1
]}

Figueiredo, Pedro ^{[2
]}

Duarte, Jose Alberto ^{[2
]}

Ferreira, Rita ^{[1
,2
]}

Amado, Francisco ^{[1
]}

机构：

[1] Univ Aveiro, Dept Quim, P-3810193 Aveiro, Portugal

[2] Univ Porto, Fac Desporto, CIAFEL, Oporto, Portugal

来源：

JOURNALS OF GERONTOLOGY SERIES A-BIOLOGICAL SCIENCES AND MEDICAL SCIENCES | 2010年 / 65卷 / 08期

关键词：

Mitochondrial aging; Exercise; Respiratory chain functionality; BN-PAGE; Two-dimensional electrophoresis; RAT-BRAIN MITOCHONDRIA; OXIDATIVE-PHOSPHORYLATION; RESPIRATORY-CHAIN; ELECTROPHORESIS; PROTEINS; EXERCISE; ANTIOXIDANTS; RESTRICTION; SARCOPENIA; COMPLEXES;

D O I：

10.1093/gerona/glq081

中图分类号：

R592 [老年病学]; C [社会科学总论];

学科分类号：

03 ; 0303 ; 100203 ;

摘要：

The aim of this study was to investigate the influence of lifestyle on the aging alterations in skeletal muscle mitochondrial proteins. Thirty C57BL/6 strain mice (2 months) were randomly divided into three groups (young, Y; old sedentary, S; and old active, A). The S and A mice were individually placed into standard cages and in cages with running wheels for 25 months. Upon killing, mitochondria from the hind limb skeletal muscles were isolated for the evaluation of general proteome alterations, carbonylation, and electron transport chain (ETC) activity. We identified 77 different proteins mostly from the oxidative phosphorylation and mitochondrial metabolism. Sedentary mice presented a significant loss of ETC functionality in opposition to active mice. Although some proteins were found damaged in both A and S mice, damage to ETC proteins was more evident in S. Moreover, it is also possible to conclude that lifestyle is a key modulator for preventing the aging-induced protein expression and functionality in mitochondria.

引用

页码：832 / 842

页数：11

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