Effects of the rol C gene on hairy root:: Induction development and tropane alkaloid production by Atropa belladonna

被引:79
作者
Bonhomme, V [1 ]
Laurain-Mattar, D [1 ]
Fliniaux, MA [1 ]
机构
[1] Fac Pharm, Lab Pharmacognosie & Phytotechnol, F-80037 Amiens, France
来源
JOURNAL OF NATURAL PRODUCTS | 2000年 / 63卷 / 09期
关键词
D O I
10.1021/np990614l
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Two series of Atropa belladonna hairy root lines were obtained, the first one transformed via Agrobacterium tumefaciens harboring rol C and npt II genes, and the other transformed with rol ABC and npt II genes. Thirteen hairy root; lines were obtained and selected on hormone-free medium. The transformation was confirmed by PCR analysis, and these root lines were first examinated for their growth rate. Then hyoscyamine and scopolamine production was measured after 3 and 4 weeks of culture to evaluate the possible role of rol C gene in tropane alkaloid formation. The rol C gene alone played a significant role in the hairy root growth rate (17-fold increase). However this effect was much lower than that induced by the rol ABC genes together (75-fold increase). In contrast, the rol C gene alone was as efficient as the rol ABC genes together (mean value of total alkaloids: 0.36% dry weight, i.e., 12-fold times more than in untransformed roots) to stimulate the biosynthesis of tropane alkaloids in A. belladonna hairy root cultures.
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页码:1249 / 1252
页数:4
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