Polyvalent Oligonucleotide Iron Oxide Nanoparticle "Click" Conjugates

被引:118
作者
Cutler, Joshua I.
Zheng, Dan
Xu, Xiaoyang
Giljohann, David A.
Mirkin, Chad A. [1 ]
机构
[1] Northwestern Univ, Dept Chem, Evanston, IL 60208 USA
关键词
DNA; click; cycloaddition; bioconjugation; nanomaterials; AZIDE-ALKYNE CYCLOADDITION; MAGNETIC NANOPARTICLES; GOLD NANOPARTICLES; DNA;
D O I
10.1021/nl100477m
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
We have utilized the copper-catalyzed azide-alkyne reaction to form a dense monolayer of oligonucleotides on a superparamagnetic nanoparticle core. These particles exhibit the canonical properties of materials densely functionalized with DNA, which can be controlled by modulating the density of oligonucleotides on the surface of the particles. Furthermore, like their Au analogues, these particles can easily cross HeLa (cervical cancer) cell membranes without transfection agents due to their dense DNA shell. Importantly, this approach should be generalizable to other azide-functionalized particles.
引用
收藏
页码:1477 / 1480
页数:4
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