Detection of dengue virus in saliva and urine by real time RT-PCR

被引：90

作者：

Poloni, Telma R. ^{[1
]}

Oliveira, Anibal S. ^{[1
]}

Alfonso, Helda L. ^{[1
]}

Galvao, Larissa R. ^{[1
]}

Amarilla, Alberto A. ^{[2
]}

Poloni, Dimair F. ^{[3
]}

Figueiredo, Luiz T. ^{[2
]}

Aquino, Victor H. ^{[1
]}

机构：

[1] Univ Sao Paulo, Dept Anal Clin Toxicol & Bromatol, Fac Ciencias Farmaceut, BR-14040903 Sao Paulo, Brazil

[2] Univ Sao Paulo, Ctr Pesquisa Virol, Fac Med Ribeirao Preto, BR-14049900 Sao Paulo, Brazil

[3] Univ Sao Paulo, Hosp Clin, Fac Med Ribeirao Preto, BR-14049900 Sao Paulo, Brazil

来源：

VIROLOGY JOURNAL | 2010年 / 7卷

基金：

巴西圣保罗研究基金会;

关键词：

INFECTION;

D O I：

10.1186/1743-422X-7-22

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Early diagnosis of dengue virus (DENV) infection is important for patient management and control of dengue outbreaks. The objective of this study was to analyze the usefulness of urine and saliva samples for early diagnosis of DENV infection by real time RT-PCR. Two febrile patients, who have been attended at the General Hospital of the School of Medicine of Ribeirao Preto, Sao Paulo University were included in the study. Serum, urine and saliva samples collected from both patients were subjected to real time RT-PCR for DENV detection and quantification. Dengue RNA was detected in serum, urine and saliva samples of both patients. Patient 1 was infected with DENV-2 and patient 2 with DENV-3. Data presented in this study suggest that urine and saliva could be used as alternative samples for early diagnosis of dengue virus infection when blood samples are difficult to obtain, e.g.,in newborns and patients with hemorrhagic syndromes.

引用

页数：4

共 10 条

[1] Genetic diversity of the E Protein of Dengue Type 3 Virus [J].

Amarilla, Alberto A. ;

de Almeida, Flavia T. ;

Jorge, Daniel M. ;

Alfonso, Helda L. ;

de Castro-Jorge, Luiza A. ;

Nogueira, Nadia A. ;

Figueiredo, Luiz T. ;

Aquino, Victor H. .

VIROLOGY JOURNAL, 2009, 6

[2] Development and evaluation of serotype- and group-specific fluorogenic reverse transcriptase PCR (TaqMan) assays for dengue virus [J].

Callahan, JD ;

Wu, SJL ;

Dion-Schultz, A ;

Mangold, BE ;

Peruski, LF ;

Watts, DM ;

Porter, KR ;

Murphy, GR ;

Suharyono, W ;

King, CC ;

Hayes, CG ;

Temenak, JJ .

JOURNAL OF CLINICAL MICROBIOLOGY, 2001, 39 (11) :4119-4124

[3] Rapid detection and quantification of RNA of Ebola and Marburg viruses, Lassa virus, Crimean-Congo hemorrhagic fever virus, Rift Valley fever virus, Dengue virus, and Yellow fever virus by real-time reverse transcription-PCR [J].

Drosten, C ;

Göttig, S ;

Schilling, S ;

Asper, M ;

Panning, M ;

Schmitz, H ;

Günther, S .

JOURNAL OF CLINICAL MICROBIOLOGY, 2002, 40 (07) :2323-2330

[4] A simple one-step real-time RT-PCR for diagnosis of dengue virus infection [J].

Godoy dos Santos, Harryson Wings ;

Ramos Silva Poloni, Telma Regina ;

Souza, Kelly Paula ;

Menjon Muller, Vanessa Danielle ;

Tremeschin, Flavia ;

Nali, Livia Christensen ;

Fantinatti, Leandro Ricardo ;

Amarilla, Alberto Anastacio ;

Alfonso Castro, Helda Liz ;

Nunes, Marcio Roberto ;

Casseb, Samir Mansour ;

Vasconcelos, Pedro Fernando ;

Badra, Soraya Jabur ;

Moraes Figueiredo, Luiz Tadeu ;

Aquino, Victor Hugo .

JOURNAL OF MEDICAL VIROLOGY, 2008, 80 (08) :1426-1433

[5]

Gubler Duane J., 2002, Trends in Microbiology, V10, P100, DOI 10.1016/S0966-842X(01)02288-0

[6]

HALSTEAD SB, 1988, SCIENCE, V239, P476, DOI 10.1126/science.3277268

[7] Dengue [J].

Halstead, Scott B. .

LANCET, 2007, 370 (9599) :1644-1652

[8] Serotype-specific detection of dengue viruses in a fourplex real-time reverse transcriptase PCR assay [J].

Johnson, BW ;

Russell, BJ ;

Lanciotti, RS .

JOURNAL OF CLINICAL MICROBIOLOGY, 2005, 43 (10) :4977-4983

[9] Confirmation of dengue virus infection by detection of dengue virus type 1 genome in urine and saliva but not in plasma [J].

Mizuno, Y. ;

Kotaki, A. ;

Harada, F. ;

Tajima, S. ;

Kurane, I. ;

Takasaki, T. .

TRANSACTIONS OF THE ROYAL SOCIETY OF TROPICAL MEDICINE AND HYGIENE, 2007, 101 (07) :738-739

[10] Kinetics of antibodies in sera, saliva, and urine samples from adult patients with primary or secondary dengue 3 virus infections [J].

Vazquez, S. ;

Cabezas, S. ;

Perez, A. B. ;

Pupo, M. ;

Ruiz, D. ;

Calzada, N. ;

Bernardo, L. ;

Castro, O. ;

Gonzalez, D. ;

Serrano, T. ;

Sanchez, A. ;

Guzman, M. G. .

INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES, 2007, 11 (03) :256-262

← 1 →