RAPD marker in grapevine (Vitis spp) similar to plant retrotransposons

被引：23

作者：

Böhm, A ^{[1
]}

Zyprian, E ^{[1
]}

机构：

[1] Fed Ctr Breeding Res Cultivated Plants, Inst Grapevine Breeding Geilweilerhof, D-76833 Siebeldingen, Germany

来源：

PLANT CELL REPORTS | 1998年 / 17卷 / 05期

关键词：

RAPD-PCR; DNA-sequencing; Vitis vinifera L; retrotransposon-like sequence;

D O I：

10.1007/s002990050417

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

When assaying for sequence heterogeneity within monomorphic RAPD-PCR products in grapevines (Vitis spp.), we observed partial digestibility of a marker with the restriction enzyme MspI. The degree of digestion varied reproducibly between different cultivars. To study this phenomenon, two of the amplification products were cloned and sequenced. They show 91.7% similarity to each other. The DNA analysed was found to possess high similarity to retrotransposons from higher plants. This element seems repetitive and dispersed throughout the genome. To our knowledge, this is the first report of retrotransposon-like elements in woody plants.

引用

页码：415 / 421

页数：7

共 34 条

[31] FINGERPRINTING GENOMES USING PCR WITH ARBITRARY PRIMERS [J].