Bioactive components of the hop strobilus: Comparison of different extraction methods by capillary electrophoretic and chromatographic methods

被引:35
作者
Helmja, Kati
Vaher, Merike
Puessa, Tonu
Kamsol, Kristjan
Orav, Anne
Kaijurand, Mihkel
机构
[1] Tallinn Univ Technol, Fac Sci, Dept Chem, EE-12618 Tallinn, Estonia
[2] Estonian Univ Life Sci, Dept Food Hyg & Control, EE-51014 Tartu, Estonia
关键词
antioxidativity; bitter acids; capillary electrophoresis; flavonoids; hop; terpenes;
D O I
10.1016/j.chroma.2006.12.067
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
In the present study, the composition of the hop strobilus extract by using different extraction methods under different solvent conditions was analysed and compared. Several separation methods were applied to obtaining detailed information about the hop extract: capillary zone electrophoresis (CZE), high-performance liquid chromatography-mass-spectrometry (HPLC-MS/MS) and gas chromatography-mass-spectrometry (GC-MS). The electropherograms of different extracts varied dramatically. The oxidation reaction of the hop strobilus extract was examined. (C) 2006 Elsevier B.V. All rights reserved.
引用
收藏
页码:222 / 229
页数:8
相关论文
共 29 条
[1]   Characterization of the methanolic extract of hops using capillary electrophoresis-electrospray ionization-mass spectrometry [J].
Arraez-Roman, David ;
Cortacero-Ramirez, Sonia ;
Segura-Carretero, Antonio ;
Martin-Lagos Contreras, Jose-Antonio ;
Fernandez-Gutierrez, Alberto .
ELECTROPHORESIS, 2006, 27 (11) :2197-2207
[2]   Hydroxyl radical in living systems and its separation methods [J].
Cheng, FC ;
Jen, JF ;
Tsai, TH .
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2002, 781 (1-2) :481-496
[3]   Analytical separation and detection methods for flavonoids [J].
de Rijke, E ;
Out, P ;
Niessen, WMA ;
Ariese, F ;
Gooijer, C ;
Brinkman, UAT .
JOURNAL OF CHROMATOGRAPHY A, 2006, 1112 (1-2) :31-63
[4]   Characterization of phenolic profiles of Northern European berries by capillary electrophoresis and determination of their antioxidant activity [J].
Ehala, S ;
Vaher, M ;
Kaljurand, M .
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2005, 53 (16) :6484-6490
[5]  
He Guo-Qing, 2005, J Zhejiang Univ Sci B, V6, P999, DOI 10.1631/jzus.2005.B0999
[6]   Flavonoid antioxidants: chemistry, metabolism and structure-activity relationships [J].
Heim, KE ;
Tagliaferro, AR ;
Bobilya, DJ .
JOURNAL OF NUTRITIONAL BIOCHEMISTRY, 2002, 13 (10) :572-584
[7]   Analysis of natural antioxidants by capillary electromigration methods [J].
Herrero, M ;
Ibáñez, E ;
Cifuentes, A .
JOURNAL OF SEPARATION SCIENCE, 2005, 28 (9-10) :883-897
[8]   Recent trends in the determination of polyphenols by electromigration methods [J].
Jác, P ;
Polásek, M ;
Pospísilová, M .
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, 2006, 40 (04) :805-814
[9]  
MORNARPERL I, 2005, J CHROMATOGR A, V1073, P201
[10]   Spectrofluorimetric determination of hydrogen peroxide scavenging activity [J].
Pazdzioch-Czochra, M ;
Widenska, A .
ANALYTICA CHIMICA ACTA, 2002, 452 (02) :177-184