Human NAT10 Is an ATP-dependent RNA Acetyltransferase Responsible for N4-Acetylcytidine Formation in 18 S Ribosomal RNA (rRNA)

被引：243

作者：

Ito, Satoshi ^{[1
]}

Horikawa, Sayuri ^{[1
]}

Suzuki, Tateki ^{[2
]}

Kawauchi, Hiroki ^{[2
]}

Tanaka, Yoshikazu ^{[2
,3
]}

Suzuki, Takeo ^{[1
]}

Suzuki, Tsutomu ^{[1
]}

机构：

[1] Univ Tokyo, Grad Sch Engn, Dept Chem & Biotechnol, Bunkyo Ku, Tokyo 1138656, Japan

[2] Hokkaido Univ, Grad Sch Life Sci, Sapporo, Hokkaido 0600810, Japan

[3] Hokkaido Univ, Fac Adv Life Sci, Sapporo, Hokkaido 0600810, Japan

来源：

JOURNAL OF BIOLOGICAL CHEMISTRY | 2014年 / 289卷 / 52期

关键词：

SMALL-SUBUNIT; PROTEIN; ACETYLATION; ANTICODON; DATABASE; CANCER;

D O I：

10.1074/jbc.C114.602698

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

Human N-acetyltransferase 10 (NAT10) is known to be a lysine acetyltransferase that targets microtubules and histones and plays an important role in cell division. NAT10 is highly expressed in malignant tumors, and is also a promising target for therapies against laminopathies and premature aging. Here we report that NAT10 is an ATP-dependent RNA acetyltransferase responsible for formation of N-4-acetylcytidine (ac(4)C) at position 1842 in the terminal helix of mammalian 18 S rRNA. RNAi-mediated knockdown of NAT10 resulted in growth retardation of human cells, and this was accompanied by high-level accumulation of the 30 S precursor of 18 S rRNA, suggesting that ac(4)C1842 formation catalyzed by NAT10 is involved in rRNA processing and ribosome biogenesis.

引用

页码：35724 / 35730

页数：7

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