Antimicrobial susceptibility of bifidobacteria

被引:119
作者
Moubareck, C
Gavini, F
Vaugien, L
Butel, MJ
Doucet-Populaire, F
机构
[1] Univ Paris 05, UFR Sci Pharmaceut & Biol, Fac Sci Pharmaceut & Biol, Microbiol Lab, F-75260 Paris 06, France
[2] Univ Toulouse 3, UFR Sci Pharmaceut, Lab bacteriol Virol & Microbiol Ind, EA 3036, F-31062 Toulouse, France
[3] INRA, Villeneuve Dascq, France
[4] Ctr Hosp Versailles, Le Chesnay, France
关键词
MICs; probiotics; anaerobes; tetracyclines; linezolid;
D O I
10.1093/jac/dkh495
中图分类号
R51 [传染病];
学科分类号
100401 ;
摘要
Objectives: The aim of our study was to analyse the antibiotic susceptibility of various strains of Bifidobacterium spp. to a wide range of antimicrobial agents. Methods: Fifty strains belonging to eight species of bifidobacteria, isolated from humans, animals or probiotic products, were tested for susceptibility to 30 antibiotics by disc diffusion on Brucella agar supplemented with 5% laked sheep blood and vitamin K1 (1 mg/L). MICs of nine anti-anaerobe agents, including three new molecules (telithromycin, linezolid and gatifloxacin), were determined using the reference agar-dilution method. Results: All strains of bifidobacteria, whatever the species, were sensitive to penicillins: penicillin G, amoxicillin (MIC50 0.06 mg/L), piperacillin, ticarcillin, imipenem and usually anti-Gram-positive antibiotics (macrolides, clindamycin, pristinamycin, vancomycin and teicoplanin). Susceptibility to cefalothin and cefotetan was variable. Most isolates (70%) were resistant to fusidic acid. As expected, high resistance rates were observed for aminoglycosides. Metronidazole, an agent known for its anti-anaerobe activity, was ineffective against 38% of the strains. The newly commercialized molecules, telithromycin, linezolid and gatifloxacin, were active with MIC50S of 1 mg/L. The only variation in susceptibility observed among the different species concerned Bifidobacterium breve, which appeared to be generally more resistant. Potentially acquired resistance was only observed against tetracycline and minocycline, in 14% of the strains. Conclusions: With regard to a general concern about the safety of probiotics, such as potential transferability of resistance determinants, bifidobacteria, with their low natural and acquired resistance to 30 antibiotics, appear risk-free.
引用
收藏
页码:38 / 44
页数:7
相关论文
共 31 条
[11]  
FULLER R, 1989, J APPL BACTERIOL, V66, P365
[12]  
Ishibashi N, 2001, AM J CLIN NUTR, V73, p465S, DOI 10.1093/ajcn/73.2.465s
[13]  
Jousimies-Somer HR, 2002, WADSWORTH KTL ANAERO, P143
[14]   A COMPARISON OF 4 METHODS FOR DETECTING BETA-LACTAMASE IN ANAEROBIC-BACTERIA [J].
LEE, DTF ;
ROSENBLATT, JE .
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE, 1983, 1 (02) :173-175
[15]   ANTIMICROBIAL SUSCEPTIBILITY OF BIFIDOBACTERIA [J].
LIM, KS ;
HUH, CS ;
BAEK, YJ .
JOURNAL OF DAIRY SCIENCE, 1993, 76 (08) :2168-2174
[16]   Probiotic Enterococcus faecium strain is a possible recipient of the vanA gene cluster [J].
Lund, B ;
Edlund, C .
CLINICAL INFECTIOUS DISEASES, 2001, 32 (09) :1384-1385
[17]   ANTI-MICROBIAL SUSCEPTIBILITY OF BIFIDOBACTERIUM [J].
MATTEUZZI, D ;
CROCIANI, F ;
BRIGIDI, P .
ANNALES DE MICROBIOLOGIE, 1983, A134 (03) :339-349
[18]  
MILLERCATCHPOLE R, 1989, BIOCH PHYSL BIFIDOBA, P177
[19]   Survey of anaerobic susceptibility patterns: A French multicentre study [J].
Mory, F ;
Lozniewski, A ;
Bland, S ;
Sedallian, A ;
Grollier, G ;
Girard-Pipau, F ;
Paris, MF ;
Dubreuil, L .
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS, 1998, 10 (03) :229-236
[20]   Multiple antibiotic resistance gene transfer from animal to human enterococci in the digestive tract of gnotobiotic mice [J].
Moubareck, C ;
Bourgeois, N ;
Courvalin, P ;
Doucet-Populaire, F .
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2003, 47 (09) :2993-2996