New approaches in GMO detection

被引:116
作者
Querci, Maddalena [1 ]
Van den Bulcke, Marc [4 ]
Zel, Jana [2 ]
Van den Eede, Guy [1 ]
Broll, Hermann [3 ]
机构
[1] Commiss European Communities, Mol Biol & Genom Unit, Inst Hlth & Consumer Protect, Joint Res Ctr, I-21027 Ispra, Va, Italy
[2] Natl Inst Biol, Dept Biotechnol & Syst Biol, Ljubljana 1000, Slovenia
[3] BfR Fed Inst Risk Assessment, Food Safety Dept, D-14195 Berlin, Germany
[4] Sci Inst Publ Hlth IPH, B-1050 Brussels, Belgium
关键词
Genetically modified organism; Genetically modified organism detection; Detection strategies; High-throughput systems; Matrix approach; Decision support systems; GENETICALLY-MODIFIED ORGANISMS; POLYMERASE-CHAIN-REACTION; SCREENING-ASSAY; MULTIPLEX-PCR; FOOD; QUANTIFICATION; VALIDATION; PLATFORM; MAIZE; ARRAY;
D O I
10.1007/s00216-009-3237-3
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
The steady rate of development and diffusion of genetically modified plants and their increasing diversification of characteristics, genes and genetic control elements poses a challenge in analysis of genetically modified organisms (GMOs). It is expected that in the near future the picture will be even more complex. Traditional approaches, mostly based on the sequential detection of one target at a time, or on a limited multiplexing, allowing only a few targets to be analysed at once, no longer meet the testing requirements. Along with new analytical technologies, new approaches for the detection of GMOs authorized for commercial purposes in various countries have been developed that rely on (1) a smart and accurate strategy for target selection, (2) the use of high-throughput systems or platforms for the detection of multiple targets and (3) algorithms that allow the conversion of analytical results into an indication of the presence of individual GMOs potentially present in an unknown sample. This paper reviews the latest progress made in GMO analysis, taking examples from the most recently developed strategies and tools, and addresses some of the critical aspects related to these approaches.
引用
收藏
页码:1991 / 2002
页数:12
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