Glycosylation of capsaicin and 8-nordihydrocapsaicin by cultured cells of Catharanthus roseus

被引：42

作者：

Shimoda, Kei

Kwon, Soonil

Utsuki, Akiko

Ohiwa, Shingo

Katsuragi, Hisashi

Yonemoto, Naoko

Hamada, Hatsuyuki

Hamada, Hiroki

机构：

[1] Okayama Univ Sci, Fac Sci, Dept Life Sci, Okayama 7000005, Japan

[2] Natl Inst Fitness & Sports Kanoya, Kagoshima 8912390, Japan

[3] Sunny Hlth Co Ltd, Chuo Ku, Tokyo 1030028, Japan

[4] Okayama Univ Sci, Fac Sci, Dept Appl Sci, Okayama 7000005, Japan

[5] Oita Univ, Fac Med, Dept Pharm & Therapeut, Oita 8795593, Japan

来源：

PHYTOCHEMISTRY | 2007年 / 68卷 / 10期

关键词：

Catharanthus roseus; apocynaceae; cultured plant cells; glycosylation; capsaicin; 8-nordihydrocapsaicin; beta-glucoside; beta-primeveroside; beta-vicianoside;

D O I：

10.1016/j.phytochem.2007.03.005

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

The glycosylation of capsaicin and 8-nordihydrocapsaicin was investigated using cultured cells of Catharanthus roseus. In addition to capsaicin 4-O-beta-D-glucopyranoside (170 mu g/g fr. wt of cells), the biotransformation products, capsaicin 4-O-(6-O-beta-D-xylopyranosyl)-beta-glucopyranoside (116 mu g/g fr. wt of cells) and capsaicin 4-O-(6-O-alpha-L-arabinopyranosyl)-beta-D-glucopyranoside (83 mu g/g fr. wt of cells), were isolated from the cell suspension after three days of incubation with capsaicin. Two other compounds, 8-nordihydrocapsaicin 4-O(6-O-beta-D-Xylopyranosyl)-beta-D-glucopyranoside (171 mu g/g fr. wt of cells) and 8-nordihydrocapsaicin 4-O-(6-O-alpha-L-arabinopyranosyl)-beta-D-glucopyranoside (122 mu/g fr. wt of cells), together with the known 8-nordihydrocapsaicin 4-O-beta-D-glucopyranoside (204 mu g/g fr. wt of cells) were also isolated from the cell suspension after incubation with 8-nordihydrocapsaicin. (C) 2007 Elsevier Ltd. All rights reserved.

引用

页码：1391 / 1396

页数：6

共 33 条

[1]

FORMATION AND METABOLISM OF THE PUNGENT PRINCIPLE OF CAPSICUM FRUITS .12. INTRACELLULAR DISTRIBUTIONS OF ENZYMES AND INTERMEDIATES INVOLVED IN BIOSYNTHESIS OF CAPSAICIN AND ITS ANALOGS IN CAPSICUM FRUITS [J].