Human T-cell leukemia virus infection of human hematopoietic progenitor cells: Maintenance of virus infection during differentiation in vitro and in vivo

被引:48
作者
Feuer, G
Fraser, JK
Zack, JA
Lee, F
Feuer, R
Chen, ISY
机构
[1] UNIV CALIF LOS ANGELES,SCH MED,DEPT MED,DIV HEMATOL ONCOL,LOS ANGELES,CA 90095
[2] UNIV CALIF LOS ANGELES,SCH MED,DEPT MICROBIOL & IMMUNOL,LOS ANGELES,CA 90095
[3] JONSSON COMPREHENS CANC CTR,LOS ANGELES,CA 90095
关键词
D O I
10.1128/JVI.70.6.4038-4044.1996
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Human T-cell leukemia virus type 1 (HTLV-1) is the etiologic agent of adult T-cell leukemia and lymphoma and HTLV-1-associated myelopathy-tropical spastic paraparesis. We examined whether HTLV could productively infect human hematopoietic progenitor cells, CD34(+) cells were enriched from human fetal liver cells and cocultivated with cell lines transformed with HTLV-1 and -2, HTLV-1 infection was established in between 10 and >95% of the enriched CD34(+) cell population, as demonstrated by quantitative PCR analysis, HTLV-1 p19 Gag expression was also detected in infected hematopoietic progenitor cells. HTLV-1-infected hematopoietic progenitor cells were cultured in semisolid medium permissive for the development of erythroid (BFU-E), myeloid (CFU-GM), and primitive progenitor (CFU-GEMM, HPP-CFC, or CFU-A) colonies, HTLV-1 sequences were detected in colonies of all hematopoietic lineages; furthermore, the ratio of HTLV genomes to the number of human cells in each infected colony was 1:1, consistent with each colony arising from a single infected hematopoietic progenitor cell, Severe combined immunodeficient mice engrafted with human fetal thymus and liver tissues (SCID-hu) develop a conjoint organ which supports human thymocyte differentiation and maturation. Inoculation of SCID-hu mice with HTLV-1-infected T cells or enriched populations of CD34(+) cells establshed viral infection of thymocytes 4 to 6 weeks postreconstitution, Thymocytes from two mice with the greatest HTLV-1 proviral burdens showed increased expression of the CD25 marker and the interleukin 2 receptor alpha chain and perturbation of CD4(+) and CD8(+) thymocyte subset distribution profiles. Hematopoietic progenitor cells and thymuses may be targets for HTLV infection in humans, and these events may play a role in the pathogenesis associated with infection.
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页码:4038 / 4044
页数:7
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