Peroxisomal membrane monocarboxylate transporters: evidence for a redox shuttle system?

被引:88
作者
McClelland, GB [1 ]
Khanna, S [1 ]
Gonzalez, GF [1 ]
Butz, CE [1 ]
Brooks, GA [1 ]
机构
[1] Univ Calif Berkeley, Dept Integrat Biol, Exercise Physiol Lab, Berkeley, CA 94720 USA
关键词
peroxisome; monocarboxylate transporter 1; monocarboxylate transporter 2; lactate shuttle; lactate dehydrogenase; beta-oxidation; liver; reduction-oxidation; pyruvate; catalase;
D O I
10.1016/S0006-291X(03)00550-3
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
One of the many functions of liver peroxisomes is the beta-oxidation of long-chain fatty acids. It is essential for the continuation of peroxisomal beta-oxidation that a redox shuttle system exist across the peroxisomal membrane to reoxidize NADH. We propose that this redox shuttle system consists of a substrate cycle between lactate and pyruvate. Here we present evidence that purified peroxisomal membranes contain both monocarboxylate transporter 1 (MCT 1) and MCT 2 and that along with peroxisomal lactate dehydrogenase (pLDH) form a Peroxisomal Lactate Shuttle. Peroxisomal beta-oxidation was greatly stimulated by the addition of pyruvate and this increase was partially inhibited by the addition of the MCT blocker alpha-cyano-4-hydroxycinnamate (CINN). We also found that peroxisomes generated lactate in the presence of pyruvate. Together these data provide compelling that the Peroxisome Lactate Shuttle helps maintain organelle redox and the proper functioning of peroxisomal P-oxidation. (C) 2003 Elsevier Science (USA). All rights reserved.
引用
收藏
页码:130 / 135
页数:6
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