High-speed electrophoretic separation of DNA fragments using a short capillary

被引：25

作者：

Chan, KC

Muschik, GM

Issaq, HJ

机构：

来源：

JOURNAL OF CHROMATOGRAPHY B | 1997年 / 695卷 / 01期

关键词：

DNA;

D O I：

10.1016/S0378-4347(97)00257-0

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Capillary electrophoresis using a replaceable gel buffer was applied to the separation of DNA fragments. A short effective length capillary (1-2 cm) at low electric field allowed the separation of a 20-1000 bp ladder in 1 min. Although similar separation speed was achieved with a longer capillary at high held, the resolution of larger fragments was degraded. The short effective length capillaries were able to separate the wildtype and mutant PCR products of the TGF-beta(1) gene in under 45 s.

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页码：113 / 115

页数：3

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