Comparative analysis of vertebrate Shh genes identifies novel conserved non-coding sequence

被引：17

作者：

Goode, DK ^{[1
]}

Snell, P ^{[1
]}

Elgar, G ^{[1
]}

机构：

[1] HGMP Resource Ctr, Comparat Genom Grp, Cambridge CB10 1SB, England

来源：

MAMMALIAN GENOME | 2003年 / 14卷 / 03期

关键词：

D O I：

10.1007/s00335-002-3052-z

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The puffer fish Takifugu rubripes (Fugu), with its compact genome, is an ideal model organism for comparative genomics. Sonic hedgehog (Shh) is a key protein in the patterning of differentiating cells during embryonic development. We have sequenced the Fugu Shh gene and compared it with the mammalian and zebrafish orthologs, identifying a number of novel conserved, non-coding sequences upstream of exon one and within the two introns. Additional conserved sequences serve to delineate activator regions and enhancers previously characterized through functional analysis. Control elements can thus be rapidly and effectively predicted by comparative methodology in its own right as well as complementing other, functional methods. This work demonstrates the value of using Fugu in comparative genomics, which has allowed identification of new putative regulatory elements, as well as corroborating enhancers identified by the more traditional deletion mapping method.

引用

页码：192 / 201

页数：10

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