Purification, composition analysis and antioxidant activity of the polysaccharides from Dendrobium nobile Lindl.

被引：245

作者：

Luo, AoXue ^{[1
,3
]}

He, XingJin ^{[1
]}

Zhou, SongDong ^{[1
]}

Fan, YiJun ^{[1
,3
]}

Luo, AoShuang ^{[2
]}

Chun, Ze ^{[2
]}

机构：

[1] Sichuan Univ, Coll Life Sci, Chengdu 610064, Peoples R China

[2] Chinese Acad Sci, Chengdu Inst Biol, Chengdu 610041, Peoples R China

[3] Sichuan Agr Univ, Dujiangyan 611830, Peoples R China

来源：

CARBOHYDRATE POLYMERS | 2010年 / 79卷 / 04期

关键词：

Dendrobium nobile Lindl; Polysaccharide; Monosaccharides composition; Antioxidant activity; CAPACITY; EXTRACTS;

D O I：

10.1016/j.carbpol.2009.10.033

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

Crude water-soluble polysaccharides were extracted from the stem of Dendrobium nobile Lindl. using boiling-water. The polysaccharides were successively purified by chromatography on DEAE-cellulose and Sephadex G-200 column, giving four major polysaccharide fractions termed DNP1-1, DNP2-1, DNP3-1, DNP4-2. The gel permeation chromatography (GPC) analysis showed that the average molecular weight (Mw) of polysaccharides (DNP1-1, DNP2-1, DNP3-1, DNP4-2) were approximately 136 kDa, 27.7 kDa, 11.8 kDa and 11.4 kDa, respectively. Monosaccharides analysis revealed the dominance of mannose, glucose, galactose, and smaller amounts of rhamnose, arabinose and xylose in the four polysaccharides. The evaluation of antioxidant activity suggested that DNP4-2 had good potential for scavenging activity of ABTS radical, and higher scavenging activity of hydroxyl radical and DPPH radical than the other polysaccharides, and should be explored as a novel potential antioxidant. (c) 2009 Elsevier Ltd. All rights reserved.

引用

页码：1014 / 1019

页数：6

共 30 条

[1]

ALOSP RM, 1982, J CHROMATOGR, V246, P227

[2] Free-radical scavenging capacity and antioxidant activity of selected plant species from the Canadian prairies [J].

Amarowicz, R ;

Pegg, RB ;

Rahimi-Moghaddam, P ;

Barl, B ;

Weil, JA .

FOOD CHEMISTRY, 2004, 84 (04) :551-562

[3] INFRA-RED SPECTRA OF CARBOHYDRATES .1. SOME DERIVATIVES OF D-GLUCOPYRANOSE [J].

BARKER, SA ;

BOURNE, EJ ;

STACEY, M ;

WHIFFEN, DH .

JOURNAL OF THE CHEMICAL SOCIETY, 1954, (JAN) :171-176

[4] Antioxidant principles from Bauhinia tarapotensis [J].

Braca, A ;

De Tommasi, N ;

Di Bari, L ;

Pizza, C ;

Politi, M ;

Morelli, I .

JOURNAL OF NATURAL PRODUCTS, 2001, 64 (07) :892-895

[5]

Chen HY, 2001, NATO SCI SER II-MATH, V13, P75

[6] Fourier transform infrared spectroscopy and chemometric analysis of white wine polysaccharide extracts [J].

Coimbra, MA ;

Gonçalves, F ;

Barros, AS ;

Delgadillo, I .

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2002, 50 (12) :3405-3411

[7] COMPARISON OF OPEN-COLUMN AND HIGH-PERFORMANCE GEL-PERMEATION CHROMATOGRAPHY IN THE SEPARATION AND MOLECULAR-WEIGHT ESTIMATION OF POLYSACCHARIDES [J].

DREHER, TW ;

HAWTHORNE, DB ;

GRANT, BR .

JOURNAL OF CHROMATOGRAPHY, 1979, 174 (02) :443-446

[8] A COLORIMETRIC METHOD FOR THE DETERMINATION OF SUGARS [J].

DUBOIS, M ;

GILLES, K ;

HAMILTON, JK ;

REBERS, PA ;

SMITH, F .

NATURE, 1951, 168 (4265) :167-167

[9] STRUCTURE OF THE CAPSULAR POLYSACCHARIDE FROM THE KLEBSIELLA K8 REFERENCE STRAIN-1015 [J].

ERBING, B ;

JANSSON, PE ;

WIDMALM, G ;

NIMMICH, W .

CARBOHYDRATE RESEARCH, 1995, 273 (02) :197-205

[10] Composition analysis and antioxidant activity of polysaccharide from Dendrobium denneanum [J].

Fan, Yijun ;

He, XingJin ;

Zhou, Songdong ;

Luo, Aoxue ;

He, Tao ;

Chun, Ze .

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 2009, 45 (02) :169-173

← 1 2 3 →