Spectroscopic study of gamma irradiated bovine hemoglobin

被引：18

作者：

Maghraby, Ahmed Mohamed

Ali, Maha Anwar

机构：

[1] Natl Inst Stand, Radiat Dosimetry Dept, Haram Giza, Egypt

[2] Cairo Univ, Fac Sci, Dept Biophys, Giza, Egypt

来源：

RADIATION PHYSICS AND CHEMISTRY | 2007年 / 76卷 / 10期

关键词：

EPR; ESR; radiation; bovine; hemoglobin;

D O I：

10.1016/j.radphyschem.2007.01.008

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

In the present study, the effects of ionizing radiation of Cs-137 and Co-60 from 4.95 to 743.14Gy and from 40Gy to 300kGy, respectively, on some bovine hemoglobin characteristics were studied. Such an effect was evaluated using electron paramagnetic resonance (EPR) spectroscopy, and infra-red (IR) spectroscopy. Bovine hemoglobin EPR spectra were recorded and analyzed before and after irradiation and changes were explained in detail. IR spectra of unirradiated and irradiated Bovine hemoglobin were recorded and analyzed also. It was found that ionizing radiation may lead to the increase of free radicals production, the decrease in a-helices contents, which reflects the degradation of hemoglobin molecular structure, or at least its incomplete performance. Results also show that the combined application of EPR and FTIR spectroscopy is a powerful tool for determining structural modification of bovine hemoglobin samples exposed to gamma irradiation. (c) 2007 Elsevier Ltd. All rights reserved.

引用

页码：1600 / 1605

页数：6

共 27 条

[1] Iron release, oxidative stress and erythrocyte ageing [J].