Microfluidic network-based combinatorial dilution device for high throughput screening and optimization

被引：34

作者：

Lee, Kangsun ^{[1
]}

Kim, Choong ^{[2
]}

Jung, Geunhui ^{[2
]}

Kim, Tae Song ^{[2
]}

Kang, Ji Yoon ^{[2
]}

Oh, Kwang W. ^{[1
]}

机构：

[1] SUNY Buffalo, SMALL Nanobio Sensors & MicroActuators Learning L, Dept Elect Engn, Buffalo, NY 14260 USA

[2] Korea Inst Sci & Technol, Nanobiores Ctr, Seoul 136791, South Korea

来源：

MICROFLUIDICS AND NANOFLUIDICS | 2010年 / 8卷 / 05期

关键词：

Combinatorial device; Microfluidic network; High throughput screening; Design of experiments; 3-DIMENSIONAL MICROCHANNEL NETWORK; GENERATION; PARALLEL; GRADIENTS; DESIGN; ARRAYS; CHIP;

D O I：

10.1007/s10404-009-0500-z

中图分类号：

TB3 [工程材料学];

学科分类号：

0805 ; 080502 ;

摘要：

We present a combinatorial dilution device using a three-layer microfluidic network that can produce systematic variations of buffer and additive solutions in a combinatorial fashion for high throughput screening and optimization. A proof-of-concept device providing seven combinations (ABC/D, AB/D, BC/D, AC/D, A/D, B/D, and C/D) of three additive samples (A, B, and C) into a buffer solution (D) has been demonstrated. Such combinations are often used in simplex-centroid mixture DOE (design of experiments), useful techniques to minimize the experimental efforts at maximal information output with systematic variations of large-scale components. Based on mathematical and electrical modeling and computational fluid dynamic simulation, the device has been designed, fabricated, and characterized.

引用

页码：677 / 685

页数：9

共 36 条

[1]

[Anonymous], ASS GUID MAN VERS 5

[2] Microfluidics-based systems biology [J].

Breslauer, DN ;

Lee, PJ ;

Lee, LP .

MOLECULAR BIOSYSTEMS, 2006, 2 (02) :97-112

[3] Generation of complex concentration profiles in microchannels in a logarithmically small number of steps [J].

Campbell, Kyle ;

Groisman, Alex .

LAB ON A CHIP, 2007, 7 (02) :264-272

[4] A multi-purpose microfluidic perfusion system with combinatorial choice of inputs, mixtures, gradient patterns, and flow rates [J].

Cooksey, Gregory A. ;

Sip, Christopher G. ;

Folch, Albert .

LAB ON A CHIP, 2009, 9 (03) :417-426

[5] Generation of gradients having complex shapes using microfluidic networks [J].

Dertinger, SKW ;

Chiu, DT ;

Jeon, NL ;

Whitesides, GM .

ANALYTICAL CHEMISTRY, 2001, 73 (06) :1240-1246

[6] Lab-on-a-chip: microfluidics in drug discovery [J].

Dittrich, PS ;

Manz, A .

NATURE REVIEWS DRUG DISCOVERY, 2006, 5 (03) :210-218

[7] Synthesis and analysis of combinatorial libraries performed in an automated micro reactor system [J].

Garcia-Egido, E ;

Spikmans, V ;

Wong, SYF ;

Warrington, BH .

LAB ON A CHIP, 2003, 3 (02) :73-76

[8] A hybrid microsystem for parallel perfusion experiments on living cells [J].

Greve, Frauke ;

Seemann, Livia ;

Hierlemann, Andreas ;

Lichtenberg, Jan .

JOURNAL OF MICROMECHANICS AND MICROENGINEERING, 2007, 17 (08) :1721-1730

[9] Generation of arbitrary monotonic concentration profiles by a serial dilution microfluidic network composed of microchannels with a high fluidic-resistance ratio [J].

Hattori, Koji ;

Sugiura, Shinji ;

Kanamori, Toshiyuki .

LAB ON A CHIP, 2009, 9 (12) :1763-1772

[10] Generating fixed concentration arrays in a microfluidic device [J].

Holden, MA ;

Kumar, S ;

Castellana, ET ;

Beskok, A ;

Cremer, PS .

SENSORS AND ACTUATORS B-CHEMICAL, 2003, 92 (1-2) :199-207

← 1 2 3 4 →