Tumor suppressor gene BRCA-1 is expressed by embryonic and adult neural stem cells and involved in cell proliferation

被引：43

作者：

Korhonen, L ^{[1
]}

Brännvall, K ^{[1
]}

Skoglösa, Y ^{[1
]}

Lindholm, D ^{[1
]}

机构：

[1] Uppsala Univ, Ctr Biomed, Dept Neurosci Neurobiol, S-75123 Uppsala, Sweden

来源：

JOURNAL OF NEUROSCIENCE RESEARCH | 2003年 / 71卷 / 06期

关键词：

BRCA-1; in situ hybridization; rat brain; neural stem cells; BrdU; cell proliferation;

D O I：

10.1002/jnr.10546

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

BRCA-1 is a tumor suppressor gene that plays a role in DNA repair and cellular growth control. Here we show that BRCA-1 mRNA is expressed by embryonic rat brain and is localized to the neuroepithelium containing neuronal precursor cells. The expression of BRCA-1 decreases during rat brain development, but BRCA-1 is expressed postnatally by proliferating neuronal precursor cells in the developing cerebellum. Neural stem cells (NSC) prepared from embryonic rat brain and cultured in the presence of epidermal growth factor were positive for BRCA-1. Induction of NSC differentiation resulted in down-regulation of BRCA-1 expression as shown by RNA and protein analyses. In addition to embryonic cells, BRCA-1 is also present in NSC prepared from adult rat brain. In adult rats, BRCA1 was expressed by cells in the walls of brain ventricles and in choroid plexus. The results show that BRCA-1 is present in embryonic and adult rat NSC and that the expression is linked to NSC proliferation. (C) 2003 Wiley-Liss, Inc.

引用

页码：769 / 776

页数：8

共 32 条

[11] Brca1 deficiency results in early embryonic lethality characterized by neuroepithelial abnormalities [J].

Gowen, LC ;

Johnson, BL ;

Latour, AM ;

Sulik, KK ;

Koller, BH .

NATURE GENETICS, 1996, 12 (02) :191-194

[12] The tumor suppressor gene Brca1 is required for embryonic cellular proliferation in the mouse [J].

Hakem, R ;

delaPompa, JL ;

Sirard, C ;

Mo, R ;

Woo, M ;

Hakem, A ;

Wakeham, A ;

Potter, J ;

Reitmair, A ;

Billia, F ;

Firpo, E ;

Hui, CC ;

Roberts, J ;

Rossant, J ;

Mak, TW .

CELL, 1996, 85 (07) :1009-1023

[13] Growth retardation and tumour inhibition by BRCA1 [J].

Holt, JT ;

Thompson, ME ;

Szabo, C ;

RobinsonBenion, C ;

Arteaga, CL ;

King, MC ;

Jensen, RA .

NATURE GENETICS, 1996, 12 (03) :298-302

[14] Identification of a neural stem cell in the adult mammalian central nervous system [J].

Johansson, CB ;

Momma, S ;

Clarke, DL ;

Risling, M ;

Lendahl, U ;

Frisén, J .

CELL, 1999, 96 (01) :25-34

[15] Expression of c-Met in developing rat hippocampus:: evidence for HGF as a neurotrophic factor for calbindin D-expressing neurons [J].

Korhonen, L ;

Sjöholm, U ;

Takei, N ;

Kern, MA ;

Schirmacher, P ;

Castrén, E ;

Lindholm, D .

EUROPEAN JOURNAL OF NEUROSCIENCE, 2000, 12 (10) :3453-3461

[16] Regulation of X-chromosome-linked inhibitor of apoptosis protein in kainic acid-induced neuronal death in the rat hippocampus [J].

Korhonen, L ;

Belluardo, N ;

Lindholm, D .

MOLECULAR AND CELLULAR NEUROSCIENCE, 2001, 17 (02) :364-372

[17] EXPRESSION OF BRCA1 IS ASSOCIATED WITH TERMINAL DIFFERENTIATION OF ECTODERMALLY AND MESODERMALLY DERIVED TISSUES IN MICE [J].

LANE, TF ;

DENG, CX ;

ELSON, A ;

LYU, MS ;

KOZAK, CA ;

LEDER, P .

GENES & DEVELOPMENT, 1995, 9 (21) :2712-2722

[18] CNS STEM-CELLS EXPRESS A NEW CLASS OF INTERMEDIATE FILAMENT PROTEIN [J].

LENDAHL, U ;

ZIMMERMAN, LB ;

MCKAY, RDG .

CELL, 1990, 60 (04) :585-595

[19] Inactivation of the mouse Brca1 gene leads to failure in the morphogenesis of the egg cylinder in early postimplantation development [J].

Liu, CY ;

FleskenNikitin, A ;

Li, S ;

Zeng, YY ;

Lee, WH .

GENES & DEVELOPMENT, 1996, 10 (14) :1835-1843

[20] Targeted mutations of breast cancer susceptibility gene homologs in mice: lethal phenotypes of Brca1, Brca2, Brca1/Brca2, Brca1/p53, and Brca2/p53 nullizygous embryos [J].

Ludwig, T ;

Chapman, DL ;

Papaioannou, VE ;

Efstratiadis, A .

GENES & DEVELOPMENT, 1997, 11 (10) :1226-1241

← 1 2 3 4 →