Tumor suppressor gene BRCA-1 is expressed by embryonic and adult neural stem cells and involved in cell proliferation

被引：43

作者：

Korhonen, L ^{[1
]}

Brännvall, K ^{[1
]}

Skoglösa, Y ^{[1
]}

Lindholm, D ^{[1
]}

机构：

[1] Uppsala Univ, Ctr Biomed, Dept Neurosci Neurobiol, S-75123 Uppsala, Sweden

来源：

JOURNAL OF NEUROSCIENCE RESEARCH | 2003年 / 71卷 / 06期

关键词：

BRCA-1; in situ hybridization; rat brain; neural stem cells; BrdU; cell proliferation;

D O I：

10.1002/jnr.10546

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

BRCA-1 is a tumor suppressor gene that plays a role in DNA repair and cellular growth control. Here we show that BRCA-1 mRNA is expressed by embryonic rat brain and is localized to the neuroepithelium containing neuronal precursor cells. The expression of BRCA-1 decreases during rat brain development, but BRCA-1 is expressed postnatally by proliferating neuronal precursor cells in the developing cerebellum. Neural stem cells (NSC) prepared from embryonic rat brain and cultured in the presence of epidermal growth factor were positive for BRCA-1. Induction of NSC differentiation resulted in down-regulation of BRCA-1 expression as shown by RNA and protein analyses. In addition to embryonic cells, BRCA-1 is also present in NSC prepared from adult rat brain. In adult rats, BRCA1 was expressed by cells in the walls of brain ventricles and in choroid plexus. The results show that BRCA-1 is present in embryonic and adult rat NSC and that the expression is linked to NSC proliferation. (C) 2003 Wiley-Liss, Inc.

引用

页码：769 / 776

页数：8

共 32 条

[21] Stem cells - hype and hope [J].

McKay, R .

NATURE, 2000, 406 (6794) :361-364

[22] NAIP interacts with hippocalcin and protects neurons against calcium-induced cell death through caspase-3-dependent and -independent pathways [J].

Mercer, EA ;

Korhonen, L ;

Skoglösa, Y ;

Olsson, PA ;

Kukkonen, JP ;

Lindholm, D .

EMBO JOURNAL, 2000, 19 (14) :3597-3607

[23] A STRONG CANDIDATE FOR THE BREAST AND OVARIAN-CANCER SUSCEPTIBILITY GENE BRCA1 [J].

MIKI, Y ;

SWENSEN, J ;

SHATTUCKEIDENS, D ;

FUTREAL, PA ;

HARSHMAN, K ;

TAVTIGIAN, S ;

LIU, QY ;

COCHRAN, C ;

BENNETT, LM ;

DING, W ;

BELL, R ;

ROSENTHAL, J ;

HUSSEY, C ;

TRAN, T ;

MCCLURE, M ;

FRYE, C ;

HATTIER, T ;

PHELPS, R ;

HAUGENSTRANO, A ;

KATCHER, H ;

YAKUMO, K ;

GHOLAMI, Z ;

SHAFFER, D ;

STONE, S ;

BAYER, S ;

WRAY, C ;

BOGDEN, R ;

DAYANANTH, P ;

WARD, J ;

TONIN, P ;

NAROD, S ;

BRISTOW, PK ;

NORRIS, FH ;

HELVERING, L ;

MORRISON, P ;

ROSTECK, P ;

LAI, M ;

BARRETT, JC ;

LEWIS, C ;

NEUHAUSEN, S ;

CANNONALBRIGHT, L ;

GOLDGAR, D ;

WISEMAN, R ;

KAMB, A ;

SKOLNICK, MH .

SCIENCE, 1994, 266 (5182) :66-71

[24] Developmental expression of Brca2 colocalizes with Brca1 and is associated with proliferation and differentiation in multiple tissues [J].

Rajan, JV ;

Marquis, ST ;

Gardner, HP ;

Chodosh, LA .

DEVELOPMENTAL BIOLOGY, 1997, 184 (02) :385-401

[25]

Rao VN, 1996, ONCOGENE, V12, P523

[26] GENERATION OF NEURONS AND ASTROCYTES FROM ISOLATED CELLS OF THE ADULT MAMMALIAN CENTRAL-NERVOUS-SYSTEM [J].

REYNOLDS, BA ;

WEISS, S .

SCIENCE, 1992, 255 (5052) :1707-1710

[27] Regulation of pituitary adenylate cyclase activating polypeptide and its receptor type 1 after traumatic brain injury:: Comparison with brain-derived neurotrophic factor and the induction of neuronal cell death [J].

Skoglösa, Y ;

Lewén, A ;

Takei, N ;

Hillered, L ;

Lindholm, D .

NEUROSCIENCE, 1999, 90 (01) :235-247

[28] Activated Notch2 signaling inhibits differentiation of cerebellar granule neuron precursors by maintaining proliferation [J].

Solecki, DJ ;

Liu, XL ;

Tomoda, T ;

Fang, Y ;

Hatten, ME .

NEURON, 2001, 31 (04) :557-568

[29] Differential induction of p53 in immature and adult rat brain following lithium-pilocarpine status epilepticus [J].

Tan, ZQ ;

Sankar, R ;

Shin, D ;

Sun, N ;

Liu, HT ;

Wasterlain, CG ;

Schreiber, SS .

BRAIN RESEARCH, 2002, 928 (1-2) :187-193

[30] BRCA1 and cell signaling [J].

Wang, Q ;

Zhang, HT ;

Fishel, R ;

Greene, MI .

ONCOGENE, 2000, 19 (53) :6152-6158

← 1 2 3 4 →