Polychlorinated biphenyls (PCBs) detection in milk samples by an electrochemical magneto-immunosensor (EMI) coupled to solid-phase extraction (SPE) and disposable low-density arrays

被引:60
作者
Centi, S.
Silva, E.
Laschi, S.
Palchetti, I.
Mascini, M.
机构
[1] Univ Florence, Dipartimento Chim, I-50019 Sesto Fiorentino, FI, Italy
[2] Cranfield Univ, Silsoe MK45 4DT, Beds, England
关键词
disposable low-density array; immunoassay; magnetic separation; polychlorinated biphenyls; solid-phase extraction; electrochemical detection;
D O I
10.1016/j.aca.2007.04.064
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
An electrochemical immunosensor for polychlorinated biphenyl (PCB) detection based on graphite screen-printed low-density arrays and on magnetic beads is reported. The immunological reaction for the detection of PCBs is based on a direct competitive assay using alkaline phosphatase (AP) as enzymatic label. After the immunochemical recognition, the modified magnetic beads are captured by a magnet on the surface of the graphite working electrode. The electrochemical detection is thus achieved through the addition of the AP substrate (alpha-naphthyl-phosphate). Two different antibodies (sIgG anti-PCB28 and rIgG anti-PCB77) were tested and compared in terms of sensitivity and ability to recognise different congeners. The developed electrochemical magneto-immunosensor (EMI) was successfully combined with solid-phase extraction (SPE) for the analysis of PCBs in milk samples. In spiked samples a recovery of 80% was obtained. The proposed strategy offers great promise for rapid, simple, cost-effective, and on-site analysis of clinical, food and environmental samples, considering also that low-density arrays allow the simultaneous analysis of different processed samples. (c) 2007 Elsevier B.V. All rights reserved.
引用
收藏
页码:9 / 16
页数:8
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