Anginex-conjugated liposomes for targeting of angiogenic endothelial cells

被引:34
作者
Brandwijk, Ricardo J. M. G. E.
Mulder, Willem J. M.
Nicolay, Klaas
Mayo, Kevin H.
Thijssen, Victor L. J. L.
Griffioen, Arjan W.
机构
[1] Maastricht Univ & Univ Hosp, Dept Pathol, Angiogenesis Lab, Res Inst Growth & Dev, NL-6202 AZ Maastricht, Netherlands
[2] Eindhoven Univ Technol, Biomed NMR, Dept Biomed Engn, NL-5600 MB Eindhoven, Netherlands
[3] Univ Minnesota, Dept Biochem Mol Biol & Biophys, Minneapolis, MN 55455 USA
关键词
D O I
10.1021/bc060316h
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Identification of a tumor angiogenesis specific ligand would allow targeting of tumor vasculature. Lipidic vehicles can be used to deliver therapeutic agents for treatment of disease or contrast agents for molecular imaging. A targeting ligand would allow specific delivery of such formulations to angiogenic sites, thereby reducing side effects and gaining efficiency. Anginex, a synthetic 33-mer angiostatic peptide, has been described to home angiogenically activated endothelium, suggesting an ideal candidate as targeting ligand. To investigate this application of anginex, fluorescently labeled paramagnetic liposomes were conjugated with anginex. Using phase contrast and fluorescence microscopy as well as magnetic resonance imaging (MRI), we demonstrate that anginex-conjugated liposomes bind specifically to activated endothelial cells, suggesting application as an angiogenesis targeting agent for molecular targeting and molecular imaging of angiogenesis-dependent disease.
引用
收藏
页码:785 / 790
页数:6
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