HPLC analysis of metabolically produced formaldehyde

被引：11

作者：

Lengyel, J

Kaláisz, H

Szarvas, I

Peltz, C

Szarkáné-Bolehovszky, A

机构：

[1] Semmelweis Univ, H-1089 Budapest, Hungary

[2] Inst Isotopes Co Ltd, Budapest, Hungary

[3] Eotvos Lorand Univ, Dept Theoret Chem, Budapest, Hungary

来源：

JOURNAL OF CHROMATOGRAPHIC SCIENCE | 2003年 / 41卷 / 04期

基金：

匈牙利科学研究基金会;

关键词：

ALIPHATIC-ALDEHYDES; N-OXIDE; ACETALDEHYDE; URINE; EXCRETION; DEPRENYL; ETHANOL; ACETONE; BLOOD;

D O I：

10.1093/chromsci/41.4.177

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Radiolabelled (-)-deprenyl is orally administered to rats, and urinary elimination of radiolabelled formaldehyde is detected. The separation is performed using high-performance liquid chromatography on octadecyl-silica stationary phase. Both the radioactivity and the UV absorbance of the dinitrophenylhydrazine formaldehyde peak are determined. Formaldehyde generation takes place by N-demethylation. Low levels of formaldehyde may have a beneficial role in counterbalancing the oxidative stress of the everyday person's life.

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页码：177 / 181

页数：5

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