Preparative isolation and purification of punicalin from pomegranate husk by high-speed countercurrent chromatography

被引：29

作者：

Zhou, Honghao ^{[1
]}

Lv, Jiao ^{[1
]}

Yuan, Qipeng ^{[1
]}

机构：

[1] Beijing Univ Chem Technol, State Key Lab Chem Resource Engn, Beijing 100029, Peoples R China

来源：

SEPARATION AND PURIFICATION TECHNOLOGY | 2010年 / 72卷 / 02期

关键词：

High-speed countercurrent chromatography; Punicalin; Pomegranate husk; ANTIOXIDANT ACTIVITY; GRANATUM; ELLAGITANNINS; TANNINS;

D O I：

10.1016/j.seppur.2010.01.018

中图分类号：

TQ [化学工业];

学科分类号：

0817 ;

摘要：

High-speed countercurrent chromatography (HSCCC) was applied to preparative separation and purification of punicalin from pomegranate husk by one-step separation. The two-phase system consisted of n-butyl alcohol-ethyl acetate-water (4:1:5, v/v/v) was employed. The revolution speed of the separation column, flow rate of the mobile phase and separation temperature were 800 rpm, 2 ml/min and 25 degrees C respectively. About 100 mg amount of crude extract was separated, yielding 13.1 mg of punicalin at a high purity of over 96%. The mass recovery was more than 90%. The product was assessed by analytical HPLC and characterized by MS, H-1 NMR and C-13 NMR. (C) 2010 Published by Elsevier B.V.

引用

页码：225 / 228

页数：4

共 13 条

[1] Pomegranate juice consumption for 3 years by patients with carotid artery stenosis reduces common carotid intima-media thickness, blood pressure and LDL oxidation [J].

Aviram, M ;

Rosenblat, M ;

Gaitini, D ;

Nitecki, S ;

Hoffman, A ;

Dornfeld, L ;

Volkova, N ;

Presser, D ;

Attias, J ;

Liker, H ;

Hayek, T .

CLINICAL NUTRITION, 2004, 23 (03) :423-433

[2] GUESS - A generally useful estimate of solvent systems in CCC [J].

Friesen, JB ;

Pauli, GF .

JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES, 2005, 28 (17) :2777-2806

[3] Multitargeted therapy of cancer by ellagitannins [J].

Heber, David .

CANCER LETTERS, 2008, 269 (02) :262-268

[4] Golden rules and pitfalls in selecting optimum conditions for high-speed counter-current chromatography [J].

Ito, Y .

JOURNAL OF CHROMATOGRAPHY A, 2005, 1065 (02) :145-168

[5] Chemopreventive and adjuvant therapeutic potential of pomegranate (Punica granatum) for human breast cancer [J].

Kim, ND ;

Mehta, R ;

Yu, WP ;

Neeman, I ;

Livney, T ;

Amichay, A ;

Poirier, D ;

Nicholls, P ;

Kirby, A ;

Jiang, WG ;

Mansel, R ;

Ramachandran, C ;

Rabi, T ;

Kaplan, B ;

Lansky, E .

BREAST CANCER RESEARCH AND TREATMENT, 2002, 71 (03) :203-217

[6] Pharmacokinetic study of ellagic acid in rat after oral administration of pomegranate leaf extract [J].

Lei, F ;

Xing, DM ;

Xiang, L ;

Zhao, YN ;

Wang, W ;

Zhang, LJ ;

Du, LJ .

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2003, 796 (01) :189-194

[7] Antioxidant and hepatoprotective effects of punicalagin and punicalin on acetaminophen-induce liver damage in rats [J].

Lin, CC ;

Hsu, YF ;

Lin, TC ;

Hsu, HY .

PHYTOTHERAPY RESEARCH, 2001, 15 (03) :206-212

[8] Two ellagitannins from the leaves of Terminalia triflora with inhibitory activity on HIV-1 reverse transcriptase [J].

Martino, V ;

Morales, J ;

Martínez-Irujo, JJ ;

Font, M ;

Monge, A ;

Coussio, J .

PHYTOTHERAPY RESEARCH, 2004, 18 (08) :667-669

[9] Studies on antioxidant activity of pomegranate (Punica granatum) peel extract using in vivo models [J].

Murthy, KNC ;

Jayaprakasha, GK ;

Singh, RP .

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2002, 50 (17) :4791-4795

[10] ANTI-AIDS AGENTS .2. INHIBITORY EFFECTS OF TANNINS ON HIV REVERSE-TRANSCRIPTASE AND HIV REPLICATION IN H9 LYMPHOCYTE-CELLS [J].

NONAKA, G ;

NISHIOKA, I ;

NISHIZAWA, M ;

YAMAGISHI, T ;

KASHIWADA, Y ;

DUTSCHMAN, GE ;

BODNER, AJ ;

KILKUSKIE, RE ;

CHENG, YC ;

LEE, KH .

JOURNAL OF NATURAL PRODUCTS, 1990, 53 (03) :587-595

← 1 2 →