Stability and catalytic properties of subtilisin in acetonitrile/dimethylformamide mixtures with low water content

被引：12

作者：

Bacheva, AV ^{[1
]}

Filippova, IY ^{[1
]}

Lysogorskaya, EN ^{[1
]}

Oksenoit, ES ^{[1
]}

机构：

[1] Moscow MV Lomonosov State Univ, Fac Chem, Moscow 119899, Russia

来源：

JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC | 2000年 / 11卷 / 2-3期

基金：

俄罗斯基础研究基金会;

关键词：

subtilisin; low water media; organic solvents; enzymatic peptide synthesis;

D O I：

10.1016/S1381-1177(00)00217-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Subtilisin 72 suspension retained high activity and stability in the triple mixtures acetonitrile/DMF/water (DMF concentration was up to 70% (v/v)). The synthetic activity of subtilisin suspension was investigated using the model ;reaction of tetrapeptide Z-Ala-Ala-Leu-Phe-pNA formation from Z-Ala-Ala-Leu-OMe and Phe-pNA. ([S] = 30 mM, [E] = 6 muM; [S]/[E] molar ratio 5000:1). In the systems containing up to 60% DMF the 95% product yield was reached within 2 h. With DMF concentration increasing to 95%, the subtilisin catalytic efficiency notably decreased, though the product yield was still 30%. In the mixture acetonitrile/80%DMF/water, the effects of enzyme concentration, the reaction time and water content on the reaction progress were studied. (C) 2000 Elsevier Science B.V. All rights reserved.

引用

页码：89 / 96

页数：8

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