Proteins entrapped in silica monoliths prepared from glyceroxysilanes

被引:49
作者
Brook, MA [1 ]
Chen, Y [1 ]
Guo, K [1 ]
Zhang, Z [1 ]
Jin, W [1 ]
Deisingh, A [1 ]
Cruz-Aguado, J [1 ]
Brennan, JD [1 ]
机构
[1] McMaster Univ, Dept Chem, Hamilton, ON L8S 4M1, Canada
基金
加拿大自然科学与工程研究理事会;
关键词
silica synthesis; biocompatible; monolithic; sugar-silanes; protein doped;
D O I
10.1023/B:JSST.0000048014.09497.39
中图分类号
TQ174 [陶瓷工业]; TB3 [工程材料学];
学科分类号
0805 ; 080502 ;
摘要
The preparation of discrete polyol based silane precursors derived from glycerol by a simple one- pot process is described. These polyol- based silanes could be hydrolyzed under mild pH conditions and upon gelation resulted in the formation of optically clear, monolithic, mesoporous silica. The hydrolysis and condensation reactions lead to cure rates that are very sensitive to ionic strength, but are almost unaffected by pH in contrast to those of alkoxysilanes derived from primary alcohols such as Si(OEt)(4). Residual glycerol in the silica monolith could be removed by washing, or could be left in the silica to reduce the magnitude of shrinkage during long term storage. The biocompatible glyceroxysilane precursors lead to materials that were able to retain the activity of entrapped enzymes over repeated cycles of use for periods of up to several months.
引用
收藏
页码:343 / 348
页数:6
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