Involvement of hepatocyte growth factor in formation of bronchoalveolar structures in embryonic rat lung in primary culture

被引：13

作者：

Itakura, A ^{[1
]}

Kurauchi, O ^{[1
]}

Morikawa, S ^{[1
]}

Okamura, M ^{[1
]}

Furugori, K ^{[1
]}

Mizutani, S ^{[1
]}

机构：

[1] Nagoya Univ, Sch Med, Dept Obstet & Gynecol, Showa Ku, Nagoya, Aichi 466, Japan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1997年 / 241卷 / 01期

关键词：

D O I：

10.1006/bbrc.1997.7526

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

To clarify the role of hepatocyte growth factor (HGF) in embryonic lung development, organoids from fetal rat lung were cultured in collagen gels with or without HGF antisense oligonucleotides. Cyst-like structures formed within 24 h in organoids isolated from fetuses after 14 days' gestation, but this was abolished by the oligonucleotide addition, apparently by interference with the endogenous expression of HGF. Electron microscopy revealed two types of structure: an alveolar type characterized by osmiophilic lamellar bodies in the cytoplasm and lumen, and a bronchial type consisting of epithelial cells bearing microvilli on their apical surfaces, HGF mRNA was detectable from day 14 in fetal lung by RT-PCR Our results suggest that HGF plays, coordinately with its expression, a crucial role in the morphogenesis of both alveolar and bronchial epithelia in the rat fetal lung. (C) 1997 Academic Press.

引用

页码：98 / 103

页数：6

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