Development of luminescent terbium(III) chelates for protein labelling: Effect of triplet-state energy level

被引：56

作者：

Takalo, H

Mukkala, VM

Merio, L

RodriguezUbis, JC

Sedano, R

Juanes, O

Brunet, E

机构：

[1] UNIV AUTONOMA MADRID,DEPT QUIM,E-28049 MADRID,SPAIN

[2] UNIV TURKU,DEPT CHEM,TURKU,FINLAND

来源：

HELVETICA CHIMICA ACTA | 1997年 / 80卷 / 02期

关键词：

D O I：

10.1002/hlca.19970800204

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The synthesis of novel Tb-III labels suitable for protein labelling are reported. Their luminescence properties as antibody conjugates were measured and compared to the results of corresponding Tb-III chelates of the parent ligand structures. When the lowest triplet-state energy level of the parent donor ligand was over 23 000 cm(-1), i. e., the energy gap between the D-5(4) level of Tb-III and the lowest triplet-state energy level of the ligand exceeded 2600 cm(-1), the label derivative with a long decay time (tau=1.35-2.93 ms) and a high luminescence yield (epsilon.Phi=3770-4560) was found to be suitable for bioaffinity assays.

引用

收藏

页码：372 / 387

页数：16

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