Development of luminescent terbium(III) chelates for protein labelling: Effect of triplet-state energy level

被引：56

作者：

Takalo, H

Mukkala, VM

Merio, L

RodriguezUbis, JC

Sedano, R

Juanes, O

Brunet, E

机构：

[1] UNIV AUTONOMA MADRID,DEPT QUIM,E-28049 MADRID,SPAIN

[2] UNIV TURKU,DEPT CHEM,TURKU,FINLAND

来源：

HELVETICA CHIMICA ACTA | 1997年 / 80卷 / 02期

关键词：

D O I：

10.1002/hlca.19970800204

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The synthesis of novel Tb-III labels suitable for protein labelling are reported. Their luminescence properties as antibody conjugates were measured and compared to the results of corresponding Tb-III chelates of the parent ligand structures. When the lowest triplet-state energy level of the parent donor ligand was over 23 000 cm(-1), i. e., the energy gap between the D-5(4) level of Tb-III and the lowest triplet-state energy level of the ligand exceeded 2600 cm(-1), the label derivative with a long decay time (tau=1.35-2.93 ms) and a high luminescence yield (epsilon.Phi=3770-4560) was found to be suitable for bioaffinity assays.

引用

收藏

页码：372 / 387

页数：16

相关论文

共 41 条

[31] TIME-RESOLVED FLUORESCENCE OF A NEW EUROPIUM CHELATE COMPLEX - DEMONSTRATION OF HIGHLY SENSITIVE DETECTION OF PROTEIN AND DNA SAMPLES [J].

SAHA, AK ;

KROSS, K ;

KLOSZEWSKI, ED ;

UPSON, DA ;

TONER, JL ;

SNOW, RA ;

BLACK, CDV ;

DESAI, VC .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1993, 115 (23) :11032-11033

[32]

SAVITSKY AP, 1995, P SOC PHOTO-OPT INS, V2388, P429, DOI 10.1117/12.208503

[33] LUMINESCENCE ENERGY-TRANSFER USING A TERBIUM CHELATE - IMPROVEMENTS ON FLUORESCENCE ENERGY-TRANSFER [J].

SELVIN, PR ;

HEARST, JE .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (21) :10024-10028

[34] LUMINESCENCE RESONANCE ENERGY-TRANSFER [J].

SELVIN, PR ;

RANA, TM ;

HEARST, JE .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1994, 116 (13) :6029-6030

[35] TIME-RESOLVED FLUORESCENCE IMAGING OF EUROPIUM CHELATE LABEL IN IMMUNOHISTOCHEMISTRY AND INSITU HYBRIDIZATION [J].

SEVEUS, L ;

VAISALA, M ;

SYRJANEN, S ;

SANDBERG, M ;

KUUSISTO, A ;

HARJU, R ;

SALO, J ;

HEMMILA, I ;

KOJOLA, H ;

SOINI, E .

CYTOMETRY, 1992, 13 (04) :329-338

[36] NEW SENSITIZER-MODIFIED CALIX[4]ARENES ENABLING NEAR-UV EXCITATION OF COMPLEXED LUMINESCENT LANTHANIDE IONS [J].

STEEMERS, FJ ;

VERBOOM, W ;

REINHOUDT, DN ;

VANDERTOL, EB ;

VERHOEVEN, JW .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1995, 117 (37) :9408-9414

[37] Synthesis and luminescence of novel Eu-III complexing agents and labels with 4-(phenylethynyl)pyridine subunits [J].

Takalo, H ;

Hemmila, I ;

Sutela, T ;

Latva, M .

HELVETICA CHIMICA ACTA, 1996, 79 (03) :789-802

[38] SYNTHESIS OF EUROPIUM(III) CHELATES SUITABLE FOR LABELING OF BIOACTIVE MOLECULES [J].

TAKALO, H ;

MUKKALA, VM ;

MIKOLA, H ;

LIITTI, P ;

HEMMILA, I .

BIOCONJUGATE CHEMISTRY, 1994, 5 (03) :278-282

[39] LUMINESCENCE OF EUROPIUM(III) CHELATES WITH 4-(ARYLETHYNYL)PYRIDINES AS LIGANDS [J].

TAKALO, H .

HELVETICA CHIMICA ACTA, 1993, 76 (02) :877-883

[40] THE INFLUENCE OF SUBSTITUENTS ON THE LUMINESCENCE PROPERTIES OF THE EU(III) AND TB(III) CHELATES OF 4-(PHENYLETHYNYL)PYRIDINE DERIVATIVES [J].

TAKALO, H ;

HANNINEN, E ;

KANKARE, J .

JOURNAL OF ALLOYS AND COMPOUNDS, 1995, 225 (1-2) :511-514

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