Synergic effect studies of the bi-enzymatic system laccase-peroxidase in a voltammetric biosensor for catecholamines

被引:62
作者
Leite, OD
Lupetti, KO
Fatibello, O
Vieira, IC
Barbosa, AD
机构
[1] Univ Fed Sao Carlos, Ctr Ciencias Exatas & Tecnol, Dept Quim, BR-13560970 Sao Carlos, SP, Brazil
[2] Univ Estadual Londrina, Dept Bioquim, BR-86051990 Londrina, PR, Brazil
[3] Univ Cuiaba, UNIC, Fac Farm & Bioquim, BR-86051990 Cuiaba, MT, Brazil
基金
巴西圣保罗研究基金会;
关键词
laccase; peroxidase; bi-enzymatic biosensor; synergic studies;
D O I
10.1016/S0039-9140(02)00650-1
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Several bi-enzymatic carbon paste biosensors modified with enzymes laccase from Pleurotus ostreatus fungi and peroxidase from zucchini (Cucurbita pepo) were constructed for evaluating the synergic effect of the two enzymes on the voltammetric biosensor response for various catecholamines. Initially was investigated the effect of pH from 5.0 to 7.5, temperature from 25 to 50 degreesC, initial stirring time from 30 to 150 s, scan rate from 10 to 60 mV s(-1) and potential pulse amplitude from 10 to 60 mV on the biosensor response for several catecholamines such as dopamine, adrenaline, isoprenaline and L-dopa. It was observed a biosensor signal increase employing both enzymes, indicating thus there is a synergic effect between laccase and peroxidase, verified also in spectrophotometric studies, in the determination of these catecholamines. (C) 2003 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:889 / 896
页数:8
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