Coherent anti-Stokes Raman scattering microscopy (CARS): Instrumentation and applications

被引：22

作者：

Djaker, Nadia

Lenne, Pierre-Francois

Marguet, Didier

Colonna, Anne

Hadjur, Christophe

Rigneault, Herve ^{[1
]}

机构：

[1] Univ Aix Marseille 3, CNRS, UMR 6133, Inst Fresnel,Mosaic Team, F-1397 Marseille 20, France

[2] Univ Mediterranee, Ctr Immunol Marseille Luminy, F-13288 Marseille, France

[3] INSERM, UMR631, F-13258 Marseille, France

[4] CNRS, UMR6102, Marseille, France

[5] LOreal Rech, Aulnay Sous Bois, France

来源：

NUCLEAR INSTRUMENTS & METHODS IN PHYSICS RESEARCH SECTION A-ACCELERATORS SPECTROMETERS DETECTORS AND ASSOCIATED EQUIPMENT | 2007年 / 571卷 / 1-2期

关键词：

nonlinear optical microscopy; four-wave mixing; chemical imaging; Raman scattering;

D O I：

10.1016/j.nima.2006.10.057

中图分类号：

TH7 [仪器、仪表];

学科分类号：

0804 ; 080401 ; 081102 ;

摘要：

Recent advances in laser physics have permitted the development of a new kind of microscopy based on stimulated Raman scattering. This new technique known as Coherent anti-Stokes Raman scattering (CARS) microscopy allows vibrational imaging with high sensitivity, high spectral resolution and three-dimensional sectioning capabilities. We review recent advances in CARS microscopy, with applications to chemical and biological systems. We also present an application of CARS microscopy with high optical resolution and spectral selectivity, in resolving structures in surface ex vivo stratum corneum by looking at the CH2 stretching vibrational band. A strong CARS signal is backscattered from an intense forward generated CARS signal in thick samples. This makes noninvasive imaging of deep structures possible, without labeling or chemical treatments. (c) 2006 Elsevier B.V. All rights reserved.

引用

页码：177 / 181

页数：5

共 21 条

[1] Ordering of water molecules between phospholipid bilayers visualized by coherent anti-Stokes Raman scattering microscopy [J].