Development of an efficient and low-cost protocol for the manual PNA synthesis by Fmoc chemistry

被引：21

作者：

Avitabile, Concetta ^{[2
]}

Moggio, Loredana ^{[1
]}

D'Andrea, Luca D. ^{[1
]}

Pedone, Carlo ^{[1
,2
]}

Romanelli, Alessandra ^{[1
,2
]}

机构：

[1] CNR, Inst Biostruct & Bioimaging, I-80134 Naples, Italy

[2] Univ Naples Federico II, Sch Biotechnol Sci, I-80134 Naples, Italy

来源：

TETRAHEDRON LETTERS | 2010年 / 51卷 / 29期

关键词：

Peptide nucleic acid; Manual synthesis; Pyridine; Fmoc; NUCLEIC-ACID; MONOMERS; CHIMERAS;

D O I：

10.1016/j.tetlet.2010.05.026

中图分类号：

O62 [有机化学];

学科分类号：

070303 [有机化学];

摘要：

An efficient and low-cost protocol for the manual synthesis of Peptide Nucleic Acids is reported here. The protocol relies on coupling reactions carried out with 2.5 equiv of PNA monomers activated with HOBT/HBTU, in the presence of pyridine/NMM. The protocol has been tested on four PNA oligomers with a length ranging from 9 to 12 bases and a purine content up to 67%. (C) 2010 Elsevier Ltd. All rights reserved.

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收藏

页码：3716 / 3718

页数：3

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