Rapid SNP diagnostics using asymmetric isothermal amplification and a new mismatch-suppression technology

被引：144

作者：

Mitani, Yasumasa

Lezhava, Alexander

Kawai, Yuki

Kikuchi, Takeshi

Oguchi-Katayama, Atsuko

Kogo, Yasushi

Itoh, Masayoshi

Miyagi, Toru

Takakura, Hideki

Hoshi, Kanako

Kato, Chiaki

Arakawa, Takahiro

Shibata, Kazuhiro

Fukui, Kenji

Masui, Ryoji

Kuramitsu, Seiki

Kiyotani, Kazuma

Chalk, Alistair

Tsunekawa, Katsuhiko

Murakami, Masami

Kamataki, Tetsuya

Oka, Takanori

Shimada, Hiroshi

Cizdziel, Paul E.

Hayashizaki, Yoshihide

机构：

[1] RIKEN, Yokohama Inst, Genome Explorat Res Grp,Tsurumi Ku, Genome Network Project Core Grp,Genom Sci Ctr, Kanagawa 2300045, Japan

[2] RIKEN, Genome Sci Lab, Discovery Res Inst, Wako Inst, Wako, Saitama 3510198, Japan

[3] Wakunaga Pharmaceut Co Ltd, Hiroshima 7391195, Japan

[4] Yokohama City Univ, Dept Surg Gastroenterol, Sch Med, Yokohama, Kanagawa 2360004, Japan

[5] Osaka Univ, Dept Biol Sci, Grad Sch Sci, Toyonaka, Osaka 5600043, Japan

[6] RIKEN, RIKEN SPring 8 Ctr, Harima Inst, Sayo, Hyogo 6795148, Japan

[7] Takasaki Univ Hlth & Welfare, Fac Pharm, Gunma 3700033, Japan

[8] Karolinska Inst, Ctr Mol Med, S-17176 Stockholm, Sweden

[9] Gunma Univ, Dept Clin Lab Med, Grad Sch Med, Maebashi, Gunma 3718511, Japan

来源：

NATURE METHODS | 2007年 / 4卷 / 03期

关键词：

D O I：

10.1038/NMETH1007

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We developed a rapid single nucleotide polymorphism (SNP) detection system named smart amplification process version 2 (SMAP 2). Because DNA amplification only occurred with a perfect primer match, amplification alone was sufficient to identify the target allele. To achieve the requisite fidelity to support this claim, we used two new and complementary approaches to suppress exponential background DNA amplification that resulted from mispriming events. SMAP 2 is isothermal and achieved SNP detection from whole human blood in 30 min when performed with a new DNA polymerase that was cloned and isolated from Alicyclobacillus acidocaldarius (Aac pol). Furthermore, to assist the scientific community in configuring SMAP 2 assays, we developed software specific for SMAP 2 primer design. With these new tools, a high-precision and rapid DNA amplification technology becomes available to aid in pharmacogenomic research and molecular-diagnostics applications.

引用

页码：257 / 262

页数：6

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