CCD camera;
digital imaging;
electron scintillator;
thin film screen;
D O I:
10.1016/S0304-3991(00)00021-8
中图分类号:
TH742 [显微镜];
学科分类号:
摘要:
A multiport-readout, frame-transfer charge-coupled device (CCD) digital imaging system has been successfully developed and tested for intermediate-high-voltage electron microscopy (IVEM) applications up to 400 keV. The system employs a back-thinned CCD with 2560 x 1960 pixels and a pixel size of 24 mu m x 24 mu m. In the current implementation, four of the eight on-chip readout ports are used in parallel each operating at a pixel rate of 1- or 2-MHz so that the entire CCD array can be read out in as short as 0.6 s. The frame-transfer readout functions as an electronic shutter which permits the rapid transfer of charges in the active pixels to four masked buffers where the charges are readout and digitized while the active area of the CCD is integrating the next frame. With a thin film-based phosphor screen and a high-performance lens relay, the system has a conversion factor of 2.1 digital units per incident electron at 400 keV, and a modulation transfer function value of 14% at the Nyquist frequency. (C) 2000 Elsevier Science B.V. All rights reserved.
机构:
Univ Calif Berkeley, Lawrence Berkeley Lab, Div Life Sci, Berkeley, CA 94720 USAUniv Calif Berkeley, Lawrence Berkeley Lab, Div Life Sci, Berkeley, CA 94720 USA
Downing, KH
;
Hendrickson, FM
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机构:
Univ Calif Berkeley, Lawrence Berkeley Lab, Div Life Sci, Berkeley, CA 94720 USAUniv Calif Berkeley, Lawrence Berkeley Lab, Div Life Sci, Berkeley, CA 94720 USA
机构:
Chinese Acad Sci, Beijing Lab Electron Microscopy, Inst Phys, Beijing 100080, Peoples R ChinaChinese Acad Sci, Beijing Lab Electron Microscopy, Inst Phys, Beijing 100080, Peoples R China
Duan, XF
;
Gao, M
论文数: 0引用数: 0
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机构:Chinese Acad Sci, Beijing Lab Electron Microscopy, Inst Phys, Beijing 100080, Peoples R China
Gao, M
;
Peng, LM
论文数: 0引用数: 0
h-index: 0
机构:Chinese Acad Sci, Beijing Lab Electron Microscopy, Inst Phys, Beijing 100080, Peoples R China
机构:
Univ Calif San Diego, Res Dept Neurosci, Ctr Res Biol Struct, Natl Ctr Microscopy & Imaging Res, La Jolla, CA 92093 USAUniv Calif San Diego, Res Dept Neurosci, Ctr Res Biol Struct, Natl Ctr Microscopy & Imaging Res, La Jolla, CA 92093 USA
Fan, GY
;
Datte, P
论文数: 0引用数: 0
h-index: 0
机构:Univ Calif San Diego, Res Dept Neurosci, Ctr Res Biol Struct, Natl Ctr Microscopy & Imaging Res, La Jolla, CA 92093 USA
Datte, P
;
Beuville, E
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机构:Univ Calif San Diego, Res Dept Neurosci, Ctr Res Biol Struct, Natl Ctr Microscopy & Imaging Res, La Jolla, CA 92093 USA
Beuville, E
;
Beche, JF
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h-index: 0
机构:Univ Calif San Diego, Res Dept Neurosci, Ctr Res Biol Struct, Natl Ctr Microscopy & Imaging Res, La Jolla, CA 92093 USA
Beche, JF
;
Millaud, J
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机构:Univ Calif San Diego, Res Dept Neurosci, Ctr Res Biol Struct, Natl Ctr Microscopy & Imaging Res, La Jolla, CA 92093 USA
Millaud, J
;
Downing, KH
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h-index: 0
机构:Univ Calif San Diego, Res Dept Neurosci, Ctr Res Biol Struct, Natl Ctr Microscopy & Imaging Res, La Jolla, CA 92093 USA
Downing, KH
;
Burkard, FT
论文数: 0引用数: 0
h-index: 0
机构:Univ Calif San Diego, Res Dept Neurosci, Ctr Res Biol Struct, Natl Ctr Microscopy & Imaging Res, La Jolla, CA 92093 USA
Burkard, FT
;
Ellisman, MH
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h-index: 0
机构:Univ Calif San Diego, Res Dept Neurosci, Ctr Res Biol Struct, Natl Ctr Microscopy & Imaging Res, La Jolla, CA 92093 USA
Ellisman, MH
;
Xuong, NH
论文数: 0引用数: 0
h-index: 0
机构:Univ Calif San Diego, Res Dept Neurosci, Ctr Res Biol Struct, Natl Ctr Microscopy & Imaging Res, La Jolla, CA 92093 USA
机构:
Univ Calif Berkeley, Lawrence Berkeley Lab, Div Life Sci, Berkeley, CA 94720 USAUniv Calif Berkeley, Lawrence Berkeley Lab, Div Life Sci, Berkeley, CA 94720 USA
Downing, KH
;
Hendrickson, FM
论文数: 0引用数: 0
h-index: 0
机构:
Univ Calif Berkeley, Lawrence Berkeley Lab, Div Life Sci, Berkeley, CA 94720 USAUniv Calif Berkeley, Lawrence Berkeley Lab, Div Life Sci, Berkeley, CA 94720 USA
机构:
Chinese Acad Sci, Beijing Lab Electron Microscopy, Inst Phys, Beijing 100080, Peoples R ChinaChinese Acad Sci, Beijing Lab Electron Microscopy, Inst Phys, Beijing 100080, Peoples R China
Duan, XF
;
Gao, M
论文数: 0引用数: 0
h-index: 0
机构:Chinese Acad Sci, Beijing Lab Electron Microscopy, Inst Phys, Beijing 100080, Peoples R China
Gao, M
;
Peng, LM
论文数: 0引用数: 0
h-index: 0
机构:Chinese Acad Sci, Beijing Lab Electron Microscopy, Inst Phys, Beijing 100080, Peoples R China
机构:
Univ Calif San Diego, Res Dept Neurosci, Ctr Res Biol Struct, Natl Ctr Microscopy & Imaging Res, La Jolla, CA 92093 USAUniv Calif San Diego, Res Dept Neurosci, Ctr Res Biol Struct, Natl Ctr Microscopy & Imaging Res, La Jolla, CA 92093 USA
Fan, GY
;
Datte, P
论文数: 0引用数: 0
h-index: 0
机构:Univ Calif San Diego, Res Dept Neurosci, Ctr Res Biol Struct, Natl Ctr Microscopy & Imaging Res, La Jolla, CA 92093 USA
Datte, P
;
Beuville, E
论文数: 0引用数: 0
h-index: 0
机构:Univ Calif San Diego, Res Dept Neurosci, Ctr Res Biol Struct, Natl Ctr Microscopy & Imaging Res, La Jolla, CA 92093 USA
Beuville, E
;
Beche, JF
论文数: 0引用数: 0
h-index: 0
机构:Univ Calif San Diego, Res Dept Neurosci, Ctr Res Biol Struct, Natl Ctr Microscopy & Imaging Res, La Jolla, CA 92093 USA
Beche, JF
;
Millaud, J
论文数: 0引用数: 0
h-index: 0
机构:Univ Calif San Diego, Res Dept Neurosci, Ctr Res Biol Struct, Natl Ctr Microscopy & Imaging Res, La Jolla, CA 92093 USA
Millaud, J
;
Downing, KH
论文数: 0引用数: 0
h-index: 0
机构:Univ Calif San Diego, Res Dept Neurosci, Ctr Res Biol Struct, Natl Ctr Microscopy & Imaging Res, La Jolla, CA 92093 USA
Downing, KH
;
Burkard, FT
论文数: 0引用数: 0
h-index: 0
机构:Univ Calif San Diego, Res Dept Neurosci, Ctr Res Biol Struct, Natl Ctr Microscopy & Imaging Res, La Jolla, CA 92093 USA
Burkard, FT
;
Ellisman, MH
论文数: 0引用数: 0
h-index: 0
机构:Univ Calif San Diego, Res Dept Neurosci, Ctr Res Biol Struct, Natl Ctr Microscopy & Imaging Res, La Jolla, CA 92093 USA
Ellisman, MH
;
Xuong, NH
论文数: 0引用数: 0
h-index: 0
机构:Univ Calif San Diego, Res Dept Neurosci, Ctr Res Biol Struct, Natl Ctr Microscopy & Imaging Res, La Jolla, CA 92093 USA