Characterization of a capillary zone electrophoresis electrospray-mass spectrometry interface

被引：16

作者：

Siethoff, C ^{[1
]}

Nigge, W ^{[1
]}

Linscheid, M ^{[1
]}

机构：

[1] ISAS Inst Spectrochem & Appl Spect, D-44013 Dortmund, Germany

来源：

ANALYTICAL CHEMISTRY | 1998年 / 70卷 / 07期

关键词：

D O I：

10.1021/ac970950b

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A dependable and stable CZE/ESI-MS interface has been constructed. To avoid instabilities in both, the capillary electrophoretic separation and the electrospray, the second of the three concentric capillaries in the three-layered sprayer has been replaced by an aluminum-coated fused-silica capillary with an inner diameter only slightly greater than the outer diameter of the separation capillary. By this means, the otherwise often observed destruction of the separation capillary ("electrodrilling") can be avoided completely due to the suppression of electrochemical processes leading to gas bubble formation at the tip of the sprayer. With some examples taken from different biochemical areas and by separation of natural compounds, the capability and the reliability of the modified sprayer as the central part of the interface are demonstrated.

引用

页码：1357 / 1361

页数：5

共 26 条

[11] ONLINE MASS-SPECTROMETRIC DETECTION FOR CAPILLARY ZONE ELECTROPHORESIS [J].

OLIVARES, JA ;

NGUYEN, NT ;

YONKER, CR ;

SMITH, RD .

ANALYTICAL CHEMISTRY, 1987, 59 (08) :1230-1232

[12] A COMPREHENSIVE STUDY OF THE LOW-ENERGY COLLISION-INDUCED DISSOCIATION OF DINUCLEOSIDE MONOPHOSPHATES [J].

PHILLIPS, DR ;

MCCLOSKEY, JA .

INTERNATIONAL JOURNAL OF MASS SPECTROMETRY AND ION PROCESSES, 1993, 128 (1-2) :61-82

[13] CAPILLARY ELECTROPHORESIS ELECTROSPRAY-IONIZATION ION-TRAP MASS-SPECTROMETRY USING A SHEATHLESS INTERFACE [J].

RAMSEY, RS ;

MCLUCKEY, SA .

JOURNAL OF MICROCOLUMN SEPARATIONS, 1995, 7 (05) :461-469

[14] LOW-ENERGY COLLISION-INDUCED DISSOCIATION OF DEPROTONATED DINUCLEOTIDES - DETERMINATION OF THE ENERGETICALLY FAVORED DISSOCIATION PATHWAYS AND THE RELATIVE ACIDITIES OF THE NUCLEIC-ACID BASES [J].

RODGERS, MT ;

CAMPBELL, S ;

MARZLUFF, EM ;

BEAUCHAMP, JL .

INTERNATIONAL JOURNAL OF MASS SPECTROMETRY AND ION PROCESSES, 1994, 137 :121-149

[15] SITE-SPECIFIC PROTONATION DIRECTS LOW-ENERGY DISSOCIATION PATHWAYS OF DINUCLEOTIDES IN THE GAS-PHASE [J].

RODGERS, MT ;

CAMPBELL, S ;

MARZLUFF, EM ;

BEAUCHAMP, JL .

INTERNATIONAL JOURNAL OF MASS SPECTROMETRY AND ION PROCESSES, 1995, 148 (1-2) :1-23

[16] Styrene oxide DNA adducts: in vitro reaction and sensitive detection of modified oligonucleotides using capillary zone electrophoresis interfaced to electrospray mass spectrometry [J].

Schrader, W ;

Linscheid, M .

ARCHIVES OF TOXICOLOGY, 1997, 71 (09) :588-595

[17] DETERMINATION OF STYRENE OXIDE ADDUCTS IN DNA AND DNA COMPONENTS [J].

SCHRADER, W ;

LINSCHEID, M .

JOURNAL OF CHROMATOGRAPHY A, 1995, 717 (1-2) :117-125

[18]

SIETHOFF C, 1996, UNPUB

[19]

SVERS JC, 1996, RAPID COMMUN MASS SP, V10, P1175

[20] A STRATEGY FOR SEQUENCING PEPTIDES FROM DILUTE MIXTURES AT THE LOW FEMTOMOLE LEVEL USING MEMBRANE PRECONCENTRATION-CAPILLARY ELECTROPHORESIS-TANDEM MASS-SPECTROMETRY (MPC-CE-MS/MS) [J].

TOMLINSON, AJ ;

NAYLOR, S .

JOURNAL OF LIQUID CHROMATOGRAPHY, 1995, 18 (18-19) :3591-3615

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