Fluorescence assay based on aptamer-quantum dot binding to Bacillus thuringiensis spores

被引:98
作者
Ikanovic, Milada
Rudzinski, Walter E.
Bruno, John G.
Allman, Amity
Carrillo, Maria P.
Dwarakanath, Sulatha
Bhahdigadi, Suneetha
Rao, Poornima
Kiel, Johnathan L.
Andrews, Carrie J.
机构
[1] Texas State Univ San Marcos, Dept Chem & Biochem, San Marcos, TX 78666 USA
[2] Operat Technol Corp, San Antonio, TX 78229 USA
[3] NanoSci Diagnost Inc, Austin, TX 78758 USA
[4] USAF, Res Lab, HEPC, Brooks AFB, TX 78235 USA
关键词
Bacillus thuringiensis; aptamer; quantum dots; SELEX; fluorescence;
D O I
10.1007/s10895-007-0158-4
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
A novel assay was developed for the detection of Bacillus thuringiensis (BT) spores. The assay is based on the fluorescence observed after binding an aptamer-quantum dot conjugate to BT spores. The in vitro selection and amplification technique called SELEX (Systematic Evolution of Ligands by EXponential enrichment) was used in order to identify the DNA aptamer sequence specific for BT. The 60 base aptamer was then coupled to fluorescent zinc sulfide-capped, cadmium selenide quantum dots (QD). The assay is semi-quantitative, specific and can detect BT at concentrations of about 1,000 colony forming units/ml.
引用
收藏
页码:193 / 199
页数:7
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