The DNA methylation events in normal and cloned rabbit embryos

被引：43

作者：

Chen, T

Zhang, YL

Jiang, Y

Liu, SZ

Schatten, H

Chen, DY

Sun, QY ^{[1
]}

机构：

[1] Chinese Acad Sci, Grad Sch, Inst Zool, State Key Lab Reprod Biol, Beijing 100080, Peoples R China

[2] Univ Missouri, Dept Vet Pathobiol, Columbia, MO 65211 USA

来源：

FEBS LETTERS | 2004年 / 578卷 / 1-2期

基金：

中国国家自然科学基金;

关键词：

DNA methylation; embryo; nuclear transfer; rabbit;

D O I：

10.1016/j.febslet.2004.10.073

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

To study the DNA methylation events in normal and cloned rabbit embryos, we investigated the methylation status of a satellite sequence and the promoter region of a single-copy gene using bisulfite-sequencing technology. During normal rabbit embryo development, both sequences maintained hypermethylation status until the 8- to 16-cell stage when progressive demethylation took place. In cloned embryos, the single-copy gene promoter sequence was rapidly demethylated and precociously de novo methylated, while the satellite sequence maintained the donor-type methylation status in all examined stages. Our results indicate that unique sequences as well as satellite sequences may have aberrant methylation patterns in cloned embryos. (C) 2004 Published by Elsevier B.V. on behalf of the Federation of European Biochemical Societies.

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收藏

页码：69 / 72

页数：4

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