Influence of the surface pressure on the organization of mixed Langmuir-Blodgett films of octadecylamine and butyrylcholinesterase. 1. Film preparation at the air-water interface

被引:27
作者
Chovelon, JM [1 ]
Wan, K
Jaffrezic-Renault, N
机构
[1] Univ Lyon 1, CNRS, LACE, UMR 5634, F-69622 Villeurbanne, France
[2] Ecole Cent Lyon, IFOS, UMR CNRS 5621, F-69131 Ecully, France
关键词
D O I
10.1021/la991048x
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
In order to obtain good biosensor characteristics, an investigation on the mixed octadecylamine/ butyrylcholinesterase monolayer at the air-water interface is conducted. The kinetics of the adsorption of the enzyme at the air-water interface is dependent on time, concentration, and the ionic interaction with the amphiphilic polar groups. From the surface pressure-molecular area isotherms and their stabilities, a model of the mixed film at the air-water interface is proposed and experimental conditions leading to a stable film are defined and explained.
引用
收藏
页码:6223 / 6227
页数:5
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