Stroke damage in mice after knocking the neutrophin-4 gene into the brain-derived neurotrophic factor locus

被引：20

作者：

Endres, M

Fan, GP

Hirt, L

Jaenisch, R

机构：

[1] Humboldt Univ, Dept Neurol, Charite Hosp, D-10117 Berlin, Germany

[2] Univ Calif Los Angeles, Sch Med, Dept Human Genet, Los Angeles, CA 90024 USA

[3] CHU Vaudois, Lab Rech Neurol, CH-1011 Lausanne, Switzerland

[4] MIT, Whitehead Inst Biomed Res, Cambridge, MA 02139 USA

来源：

JOURNAL OF CEREBRAL BLOOD FLOW AND METABOLISM | 2003年 / 23卷 / 02期

关键词：

BDNF; cerebral ischemia; knock-in; neurotrophins; NT4;

D O I：

10.1097/01.WCB.0000043949.67811.C6

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Neurotrophins play a protective role during cerebral ischemia, and mice lacking both alleles for neurotrophin 4 (Nt4(-/-)) or deficient in a single allele for brain-derived neurotrophic factor (Bduf(+/-)) have increased susceptibility to cerebral ischemia. This study directly compared the biologic activities of brain-derived neurotrophic factor (BDNF) and NT4 by replacing the Bdnf coding sequence with the Nt4 sequence (Bdnf(+/nt4-ki)). Mice expressing one Nt4 allele in place of Bdnf develop 61% bigger lesions after 1-hour middle cerebral artery occlusion compared with wild-type littermates. Physiologic parameters did not contribute to ischemia susceptibility. In conclusion, NT4 is less potent than BDNF in promoting brain survival after stroke.

引用

页码：150 / 153

页数：4

共 20 条

[1] BRAIN-DERIVED NEUROTROPHIC FACTOR PROTECTS AGAINST ISCHEMIC CELL-DAMAGE IN RAT HIPPOCAMPUS [J].