Semiquantitative Study of the EDC/NHS Activation of Acid Terminal Groups at Modified Porous Silicon Surfaces

被引：308

作者：

Sam, S. ^{[1
,2
,3
]}

Touahir, L. ^{[1
]}

Andresa, J. Salvador ^{[1
]}

Allongue, P. ^{[1
]}

Chazalviel, J. -N. ^{[1
]}

Gouget-Laemmel, A. C. ^{[1
]}

de Villeneuve, C. Henry ^{[1
]}

Moraillon, A. ^{[1
]}

Ozanam, F. ^{[1
]}

Gabouze, N. ^{[2
]}

Djebbar, S. ^{[3
]}

机构：

[1] Ecole Polytech, CNRS, F-91128 Palaiseau, France

[2] UDTS, Algiers, Algeria

[3] USTHB, Fac Chim, Lab Hydromet & Chim Inorgan Mol, Algiers 16111, Algeria

来源：

LANGMUIR | 2010年 / 26卷 / 02期

关键词：

SELF-ASSEMBLED MONOLAYERS; ALKYL MONOLAYERS; PROTEIN IMMOBILIZATION; CARBOXYLIC-ACID; DNA; HYDROGEN; HYDROSILYLATION; ESTER; GOLD; FUNCTIONALIZATION;

D O I：

10.1021/la902220a

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Infrared spectroscopy is used to investigate the transformation of carboxyl-terminated alkyl chains immobilized on a surface into succinimidyl ester-terminated chains by reaction with an aqueous solution of N-ethyl-N'-(3-(dimethylamino)propyl)carbodiimide (EDC) and N-hydroxysuccinimide (NHS). The acid chains are covalently grafted at the surface of hydrogenated porous silicon whose large specific surface area allows for assessing the activation yield in a semiquantitative way by infrared (IR) spectroscopy and detecting trace amounts of surface products and/or reaction products of small IR cross section. In this way, we rationalize the different reaction paths and optimize the reaction conditions to obtain as pure as possible succinimidyl ester-terminated surfaces. A diagram mapping the surface composition after activation was constructed by systematically varying the solution composition. Results are accounted for by NHS surface adsorption and a kinetic competition between the various EDC-induced surface reactions.

引用

页码：809 / 814

页数：6

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