Tetracycline resistance genes in activated sludge wastewater treatment plants

被引：485

作者：

Auerbach, Eric A. ^{[1
]}

Seyfried, Erin E. ^{[1
]}

McMahon, Katherine D. ^{[1
]}

机构：

[1] Univ Wisconsin, Dept Civil & Environm Engn, Madison, WI 53706 USA

来源：

WATER RESEARCH | 2007年 / 41卷 / 05期

关键词：

tetracycline resistance; antibiotics; activated sludge; PCR;

D O I：

10.1016/j.watres.2006.11.045

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

The development and proliferation of antibiotic resistance in pathogenic, commensal, and environmental microorganisms is a major public health concern. The extent to which human activities contribute to the maintenance of environmental reservoirs of antibiotic resistance is poorly understood. In the current study, wastewater treatment plants (WWTPs) were investigated as possible sources of tetracycline resistance via qualitative PCR and quantitative PCR (qPCR). Various WWTPs and two freshwater lakes were surveyed for the presence of an array of 10 tetracycline resistance determinants (tet(R)): tet(A)-(E), tet(G), tet(M), tet(O), tet(Q), tet(S). All WWTP samples contained more different types of tetR genes, as compared to the lake water samples. Gene copy numbers of tet(G) and tet(Q) in these samples were quantified via qPCR and normalized to both the volume of original sample and to the amount of DNA extracted per sample (a proxy for bacterial abundance). Concentrations of tet(Q) were found to be highest in wastewater influent while tet(G) concentrations were highest in activated sludge. Investigation of the effects of LTV disinfection on wastewater effluent showed no reduction in the number of detectable tetR gene types. (C) 2007 Elsevier Ltd. All rights reserved.

引用

页码：1143 / 1151

页数：9

共 31 条

[11] Classification of antibiotic resistance patterns of indicator bacteria by discriminant analysis: Use in predicting the source of fecal contamination in subtropical waters [J].

Harwood, VJ ;

Whitlock, J ;

Withington, V .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2000, 66 (09) :3698-3704

[12]

Isaacson RE, 2002, ROLE ANTIBIOTICS AGR

[13] Pharmaceuticals, hormones, and other organic wastewater contaminants in US streams, 1999-2000: A national reconnaissance [J].

Kolpin, DW ;

Furlong, ET ;

Meyer, MT ;

Thurman, EM ;

Zaugg, SD ;

Barber, LB ;

Buxton, HT .

ENVIRONMENTAL SCIENCE & TECHNOLOGY, 2002, 36 (06) :1202-1211

[14]

Levy S., 2002, ANTIBIOTIC PARADOX M

[15] Nomenclature for new tetracycline resistance determinants [J].

Levy, SB ;

McMurry, LM ;

Barbosa, TM ;

Burdett, V ;

Courvalin, P ;

Hillen, W ;

Roberts, MC ;

Rood, JI ;

Taylor, DE .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1999, 43 (06) :1523-1524

[16] LOCATION AND CHARACTERISTICS OF THE TRANSFER REGION OF A BACTEROIDES CONJUGATIVE TRANSPOSON AND REGULATION OF TRANSFER GENES [J].

LI, LY ;

SHOEMAKER, NB ;

SALYERS, AA .

JOURNAL OF BACTERIOLOGY, 1995, 177 (17) :4992-4999

[17] Analysis of trace levels of sulfonamide and tetracycline antimicrobials, in groundwater and surface water using solid-phase extraction and liquid chromatography/mass spectrometry [J].

Lindsey, ME ;

Meyer, M ;

Thurman, EM .

ANALYTICAL CHEMISTRY, 2001, 73 (19) :4640-4646

[18] ANTIBIOTIC-RESISTANT GRAM-NEGATIVE BACTERIA IN RURAL GROUNDWATER SUPPLIES [J].

MCKEON, DM ;

CALABRESE, JP ;

BISSONNETTE, GK .

WATER RESEARCH, 1995, 29 (08) :1902-1908

[19] Genetic characterization of antimicrobial resistance in Canadian isolates of Salmonella serovar typhimurium DT104 [J].

Ng, LK ;

Mulvey, MR ;

Martin, I ;

Peters, GA ;

Johnson, W .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1999, 43 (12) :3018-3021

[20] Multiplex PCR for the detection of tetracycline resistant genes [J].

Ng, LK ;

Martin, I ;

Alfa, M ;

Mulvey, M .

MOLECULAR AND CELLULAR PROBES, 2001, 15 (04) :209-215

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