共 150 条
Quantitative Proteomics by Metabolic Labeling of Model Organisms
被引:111
作者:

Gouw, Joost W.
论文数: 0 引用数: 0
h-index: 0
机构: Univ Utrecht, Biomol Mass Spectrometry & Prote Grp, Bijvoet Ctr Biomol Res, NL-3584 CH Utrecht, Netherlands

Krijgsveld, Jeroen
论文数: 0 引用数: 0
h-index: 0
机构: Univ Utrecht, Biomol Mass Spectrometry & Prote Grp, Bijvoet Ctr Biomol Res, NL-3584 CH Utrecht, Netherlands

Heck, Albert J. R.
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Utrecht, Biomol Mass Spectrometry & Prote Grp, Bijvoet Ctr Biomol Res, NL-3584 CH Utrecht, Netherlands Univ Utrecht, Biomol Mass Spectrometry & Prote Grp, Bijvoet Ctr Biomol Res, NL-3584 CH Utrecht, Netherlands
机构:
[1] Univ Utrecht, Biomol Mass Spectrometry & Prote Grp, Bijvoet Ctr Biomol Res, NL-3584 CH Utrecht, Netherlands
关键词:
EFFICIENT PROTEIN IDENTIFICATION;
DESORPTION/IONIZATION MASS-SPECTROMETRY;
REVEALS POSTTRANSCRIPTIONAL REGULATION;
SITE-SPECIFIC PHOSPHORYLATION;
CELL-CULTURE SILAC;
STABLE-ISOTOPE;
DROSOPHILA-MELANOGASTER;
ARABIDOPSIS-THALIANA;
SACCHAROMYCES-CEREVISIAE;
IN-VIVO;
D O I:
10.1074/mcp.R900001-MCP200
中图分类号:
Q5 [生物化学];
学科分类号:
071010 ;
081704 ;
摘要:
In the biological sciences, model organisms have been used for many decades and have enabled the gathering of a large proportion of our present day knowledge of basic biological processes and their derailments in disease. Although in many of these studies using model organisms, the focus has primarily been on genetics and genomics approaches, it is important that methods become available to extend this to the relevant protein level. Mass spectrometry-based proteomics is increasingly becoming the standard to comprehensively analyze proteomes. An important transition has been made recently by moving from charting static proteomes to monitoring their dynamics by simultaneously quantifying multiple proteins obtained from differently treated samples. Especially the labeling with stable isotopes has proved an effective means to accurately determine differential expression levels of proteins. Among these, metabolic incorporation of stable isotopes in vivo in whole organisms is one of the favored strategies. In this perspective, we will focus on methodologies to stable isotope label a variety of model organisms in vivo, ranging from relatively simple organisms such as bacteria and yeast to Caenorhabditis elegans, Drosophila, and Arabidopsis up to mammals such as rats and mice. We also summarize how this has opened up ways to investigate biological processes at the protein level in health and disease, revealing conservation and variation across the evolutionary tree of life. Molecular & Cellular Proteomics 9: 11-24, 2010.
引用
收藏
页码:11 / 24
页数:14
相关论文
共 150 条
[1]
The genome sequence of Drosophila melanogaster
[J].
Adams, MD
;
Celniker, SE
;
Holt, RA
;
Evans, CA
;
Gocayne, JD
;
Amanatides, PG
;
Scherer, SE
;
Li, PW
;
Hoskins, RA
;
Galle, RF
;
George, RA
;
Lewis, SE
;
Richards, S
;
Ashburner, M
;
Henderson, SN
;
Sutton, GG
;
Wortman, JR
;
Yandell, MD
;
Zhang, Q
;
Chen, LX
;
Brandon, RC
;
Rogers, YHC
;
Blazej, RG
;
Champe, M
;
Pfeiffer, BD
;
Wan, KH
;
Doyle, C
;
Baxter, EG
;
Helt, G
;
Nelson, CR
;
Miklos, GLG
;
Abril, JF
;
Agbayani, A
;
An, HJ
;
Andrews-Pfannkoch, C
;
Baldwin, D
;
Ballew, RM
;
Basu, A
;
Baxendale, J
;
Bayraktaroglu, L
;
Beasley, EM
;
Beeson, KY
;
Benos, PV
;
Berman, BP
;
Bhandari, D
;
Bolshakov, S
;
Borkova, D
;
Botchan, MR
;
Bouck, J
;
Brokstein, P
.
SCIENCE,
2000, 287 (5461)
:2185-2195

Adams, MD
论文数: 0 引用数: 0
h-index: 0
机构: Celera Genom, Rockville, MD 20850 USA

Celniker, SE
论文数: 0 引用数: 0
h-index: 0
机构: Celera Genom, Rockville, MD 20850 USA

Holt, RA
论文数: 0 引用数: 0
h-index: 0
机构: Celera Genom, Rockville, MD 20850 USA

Evans, CA
论文数: 0 引用数: 0
h-index: 0
机构: Celera Genom, Rockville, MD 20850 USA

Gocayne, JD
论文数: 0 引用数: 0
h-index: 0
机构: Celera Genom, Rockville, MD 20850 USA

Amanatides, PG
论文数: 0 引用数: 0
h-index: 0
机构: Celera Genom, Rockville, MD 20850 USA

Scherer, SE
论文数: 0 引用数: 0
h-index: 0
机构: Celera Genom, Rockville, MD 20850 USA

Li, PW
论文数: 0 引用数: 0
h-index: 0
机构: Celera Genom, Rockville, MD 20850 USA

Hoskins, RA
论文数: 0 引用数: 0
h-index: 0
机构: Celera Genom, Rockville, MD 20850 USA

Galle, RF
论文数: 0 引用数: 0
h-index: 0
机构: Celera Genom, Rockville, MD 20850 USA

George, RA
论文数: 0 引用数: 0
h-index: 0
机构: Celera Genom, Rockville, MD 20850 USA

Lewis, SE
论文数: 0 引用数: 0
h-index: 0
机构: Celera Genom, Rockville, MD 20850 USA

Richards, S
论文数: 0 引用数: 0
h-index: 0
机构: Celera Genom, Rockville, MD 20850 USA

Ashburner, M
论文数: 0 引用数: 0
h-index: 0
机构: Celera Genom, Rockville, MD 20850 USA

Henderson, SN
论文数: 0 引用数: 0
h-index: 0
机构: Celera Genom, Rockville, MD 20850 USA

Sutton, GG
论文数: 0 引用数: 0
h-index: 0
机构: Celera Genom, Rockville, MD 20850 USA

Wortman, JR
论文数: 0 引用数: 0
h-index: 0
机构: Celera Genom, Rockville, MD 20850 USA

Yandell, MD
论文数: 0 引用数: 0
h-index: 0
机构: Celera Genom, Rockville, MD 20850 USA

Zhang, Q
论文数: 0 引用数: 0
h-index: 0
机构: Celera Genom, Rockville, MD 20850 USA

Chen, LX
论文数: 0 引用数: 0
h-index: 0
机构: Celera Genom, Rockville, MD 20850 USA

Brandon, RC
论文数: 0 引用数: 0
h-index: 0
机构: Celera Genom, Rockville, MD 20850 USA

Rogers, YHC
论文数: 0 引用数: 0
h-index: 0
机构: Celera Genom, Rockville, MD 20850 USA

Blazej, RG
论文数: 0 引用数: 0
h-index: 0
机构: Celera Genom, Rockville, MD 20850 USA

Champe, M
论文数: 0 引用数: 0
h-index: 0
机构: Celera Genom, Rockville, MD 20850 USA

Pfeiffer, BD
论文数: 0 引用数: 0
h-index: 0
机构: Celera Genom, Rockville, MD 20850 USA

Wan, KH
论文数: 0 引用数: 0
h-index: 0
机构: Celera Genom, Rockville, MD 20850 USA

Doyle, C
论文数: 0 引用数: 0
h-index: 0
机构: Celera Genom, Rockville, MD 20850 USA

Baxter, EG
论文数: 0 引用数: 0
h-index: 0
机构: Celera Genom, Rockville, MD 20850 USA

Helt, G
论文数: 0 引用数: 0
h-index: 0
机构: Celera Genom, Rockville, MD 20850 USA

Nelson, CR
论文数: 0 引用数: 0
h-index: 0
机构: Celera Genom, Rockville, MD 20850 USA

Miklos, GLG
论文数: 0 引用数: 0
h-index: 0
机构: Celera Genom, Rockville, MD 20850 USA

Abril, JF
论文数: 0 引用数: 0
h-index: 0
机构: Celera Genom, Rockville, MD 20850 USA

Agbayani, A
论文数: 0 引用数: 0
h-index: 0
机构: Celera Genom, Rockville, MD 20850 USA

An, HJ
论文数: 0 引用数: 0
h-index: 0
机构: Celera Genom, Rockville, MD 20850 USA

Andrews-Pfannkoch, C
论文数: 0 引用数: 0
h-index: 0
机构: Celera Genom, Rockville, MD 20850 USA

Baldwin, D
论文数: 0 引用数: 0
h-index: 0
机构: Celera Genom, Rockville, MD 20850 USA

Ballew, RM
论文数: 0 引用数: 0
h-index: 0
机构: Celera Genom, Rockville, MD 20850 USA

Basu, A
论文数: 0 引用数: 0
h-index: 0
机构: Celera Genom, Rockville, MD 20850 USA

Baxendale, J
论文数: 0 引用数: 0
h-index: 0
机构: Celera Genom, Rockville, MD 20850 USA

Bayraktaroglu, L
论文数: 0 引用数: 0
h-index: 0
机构: Celera Genom, Rockville, MD 20850 USA

Beasley, EM
论文数: 0 引用数: 0
h-index: 0
机构: Celera Genom, Rockville, MD 20850 USA

Beeson, KY
论文数: 0 引用数: 0
h-index: 0
机构: Celera Genom, Rockville, MD 20850 USA

Benos, PV
论文数: 0 引用数: 0
h-index: 0
机构: Celera Genom, Rockville, MD 20850 USA

Berman, BP
论文数: 0 引用数: 0
h-index: 0
机构: Celera Genom, Rockville, MD 20850 USA

Bhandari, D
论文数: 0 引用数: 0
h-index: 0
机构: Celera Genom, Rockville, MD 20850 USA

Bolshakov, S
论文数: 0 引用数: 0
h-index: 0
机构: Celera Genom, Rockville, MD 20850 USA

Borkova, D
论文数: 0 引用数: 0
h-index: 0
机构: Celera Genom, Rockville, MD 20850 USA

Botchan, MR
论文数: 0 引用数: 0
h-index: 0
机构: Celera Genom, Rockville, MD 20850 USA

Bouck, J
论文数: 0 引用数: 0
h-index: 0
机构: Celera Genom, Rockville, MD 20850 USA

Brokstein, P
论文数: 0 引用数: 0
h-index: 0
机构: Celera Genom, Rockville, MD 20850 USA
[2]
Mass spectrometry-based proteomics
[J].
Aebersold, R
;
Mann, M
.
NATURE,
2003, 422 (6928)
:198-207

Aebersold, R
论文数: 0 引用数: 0
h-index: 0
机构: Inst Syst Biol, Seattle, WA 98103 USA

Mann, M
论文数: 0 引用数: 0
h-index: 0
机构: Inst Syst Biol, Seattle, WA 98103 USA
[3]
Nucleolar proteome dynamics
[J].
Andersen, JS
;
Lam, YW
;
Leung, AKL
;
Ong, SE
;
Lyon, CE
;
Lamond, AI
;
Mann, M
.
NATURE,
2005, 433 (7021)
:77-83

Andersen, JS
论文数: 0 引用数: 0
h-index: 0
机构: Univ Dundee, Wellcome Trust Bioctr, Dundee DD1 4HN, Scotland

Lam, YW
论文数: 0 引用数: 0
h-index: 0
机构: Univ Dundee, Wellcome Trust Bioctr, Dundee DD1 4HN, Scotland

Leung, AKL
论文数: 0 引用数: 0
h-index: 0
机构: Univ Dundee, Wellcome Trust Bioctr, Dundee DD1 4HN, Scotland

Ong, SE
论文数: 0 引用数: 0
h-index: 0
机构: Univ Dundee, Wellcome Trust Bioctr, Dundee DD1 4HN, Scotland

Lyon, CE
论文数: 0 引用数: 0
h-index: 0
机构: Univ Dundee, Wellcome Trust Bioctr, Dundee DD1 4HN, Scotland

Lamond, AI
论文数: 0 引用数: 0
h-index: 0
机构: Univ Dundee, Wellcome Trust Bioctr, Dundee DD1 4HN, Scotland

Mann, M
论文数: 0 引用数: 0
h-index: 0
机构: Univ Dundee, Wellcome Trust Bioctr, Dundee DD1 4HN, Scotland
[4]
The versatile worm:: genetic and genomic resources for Caenorhabditis elegans research
[J].
Antoshechkin, Igor
;
Sternberg, Paul W.
.
NATURE REVIEWS GENETICS,
2007, 8 (07)
:518-532

Antoshechkin, Igor
论文数: 0 引用数: 0
h-index: 0
机构: CALTECH, Div Biol 156 29, Pasadena, CA 91125 USA

Sternberg, Paul W.
论文数: 0 引用数: 0
h-index: 0
机构: CALTECH, Div Biol 156 29, Pasadena, CA 91125 USA
[5]
Quantitative proteomics as a new piece of the systems biology puzzle
[J].
Bachi, Angela
;
Bonaldi, Tiziana
.
JOURNAL OF PROTEOMICS,
2008, 71 (03)
:357-367

Bachi, Angela
论文数: 0 引用数: 0
h-index: 0
机构:
Ist Sci San Raffaele, DIBIT, I-20132 Milan, Italy European Inst Oncol, Dept Expt Oncol, I-20139 Milan, Italy

Bonaldi, Tiziana
论文数: 0 引用数: 0
h-index: 0
机构:
European Inst Oncol, Dept Expt Oncol, I-20139 Milan, Italy European Inst Oncol, Dept Expt Oncol, I-20139 Milan, Italy
[6]
Quantitative mass spectrometry in proteomics: a critical review
[J].
Bantscheff, Marcus
;
Schirle, Markus
;
Sweetman, Gavain
;
Rick, Jens
;
Kuster, Bernhard
.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY,
2007, 389 (04)
:1017-1031

Bantscheff, Marcus
论文数: 0 引用数: 0
h-index: 0
机构:
Cellzome AG, D-69254 Heidelberg, Germany Cellzome AG, D-69254 Heidelberg, Germany

Schirle, Markus
论文数: 0 引用数: 0
h-index: 0
机构:
Cellzome AG, D-69254 Heidelberg, Germany Cellzome AG, D-69254 Heidelberg, Germany

Sweetman, Gavain
论文数: 0 引用数: 0
h-index: 0
机构:
Cellzome AG, D-69254 Heidelberg, Germany Cellzome AG, D-69254 Heidelberg, Germany

Rick, Jens
论文数: 0 引用数: 0
h-index: 0
机构:
Cellzome AG, D-69254 Heidelberg, Germany Cellzome AG, D-69254 Heidelberg, Germany

Kuster, Bernhard
论文数: 0 引用数: 0
h-index: 0
机构:
Cellzome AG, D-69254 Heidelberg, Germany Cellzome AG, D-69254 Heidelberg, Germany
[7]
Prevention of amino acid conversion in SILAC experiments with embryonic stem cells
[J].
Bendall, Sean C.
;
Hughes, Chris
;
Stewart, Morag H.
;
Doble, Brad
;
Bhatia, Mickie
;
Lajoie, Gilles A.
.
MOLECULAR & CELLULAR PROTEOMICS,
2008, 7 (09)
:1587-1597

Bendall, Sean C.
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Western Ontario, Don Rix Prot Identificat Facil, Dept Biochem, Schulich Sch Med & Dent, London, ON N6A 5C1, Canada
McMaster Univ, Stem Cell & Canc Res Inst, Michael G DeGroote Sch Med, Fac Hlth Sci, Hamilton, ON L9G 4L6, Canada Univ Western Ontario, Don Rix Prot Identificat Facil, Dept Biochem, Schulich Sch Med & Dent, London, ON N6A 5C1, Canada

Hughes, Chris
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Western Ontario, Don Rix Prot Identificat Facil, Dept Biochem, Schulich Sch Med & Dent, London, ON N6A 5C1, Canada Univ Western Ontario, Don Rix Prot Identificat Facil, Dept Biochem, Schulich Sch Med & Dent, London, ON N6A 5C1, Canada

Stewart, Morag H.
论文数: 0 引用数: 0
h-index: 0
机构:
McMaster Univ, Stem Cell & Canc Res Inst, Michael G DeGroote Sch Med, Fac Hlth Sci, Hamilton, ON L9G 4L6, Canada Univ Western Ontario, Don Rix Prot Identificat Facil, Dept Biochem, Schulich Sch Med & Dent, London, ON N6A 5C1, Canada

Doble, Brad
论文数: 0 引用数: 0
h-index: 0
机构:
McMaster Univ, Stem Cell & Canc Res Inst, Michael G DeGroote Sch Med, Fac Hlth Sci, Hamilton, ON L9G 4L6, Canada Univ Western Ontario, Don Rix Prot Identificat Facil, Dept Biochem, Schulich Sch Med & Dent, London, ON N6A 5C1, Canada

Bhatia, Mickie
论文数: 0 引用数: 0
h-index: 0
机构:
McMaster Univ, Stem Cell & Canc Res Inst, Michael G DeGroote Sch Med, Fac Hlth Sci, Hamilton, ON L9G 4L6, Canada Univ Western Ontario, Don Rix Prot Identificat Facil, Dept Biochem, Schulich Sch Med & Dent, London, ON N6A 5C1, Canada

Lajoie, Gilles A.
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Western Ontario, Don Rix Prot Identificat Facil, Dept Biochem, Schulich Sch Med & Dent, London, ON N6A 5C1, Canada Univ Western Ontario, Don Rix Prot Identificat Facil, Dept Biochem, Schulich Sch Med & Dent, London, ON N6A 5C1, Canada
[8]
Quantitative phosphoproteomics of early elicitor signaling in Arabidopsis
[J].
Benschop, Joris J.
;
Mohammed, Shabaz
;
O'Flaherty, Martina
;
Heck, Albert J. R.
;
Slijper, Monique
;
Menke, Frank L. H.
.
MOLECULAR & CELLULAR PROTEOMICS,
2007, 6 (07)
:1198-1214

Benschop, Joris J.
论文数: 0 引用数: 0
h-index: 0
机构: Univ Utrecht, Dept Biomol Mass Spectrometry, Bijovet Ctr Biomed Res, NL-3584 CH Utrecht, Netherlands

Mohammed, Shabaz
论文数: 0 引用数: 0
h-index: 0
机构: Univ Utrecht, Dept Biomol Mass Spectrometry, Bijovet Ctr Biomed Res, NL-3584 CH Utrecht, Netherlands

O'Flaherty, Martina
论文数: 0 引用数: 0
h-index: 0
机构: Univ Utrecht, Dept Biomol Mass Spectrometry, Bijovet Ctr Biomed Res, NL-3584 CH Utrecht, Netherlands

Heck, Albert J. R.
论文数: 0 引用数: 0
h-index: 0
机构: Univ Utrecht, Dept Biomol Mass Spectrometry, Bijovet Ctr Biomed Res, NL-3584 CH Utrecht, Netherlands

Slijper, Monique
论文数: 0 引用数: 0
h-index: 0
机构: Univ Utrecht, Dept Biomol Mass Spectrometry, Bijovet Ctr Biomed Res, NL-3584 CH Utrecht, Netherlands

Menke, Frank L. H.
论文数: 0 引用数: 0
h-index: 0
机构: Univ Utrecht, Dept Biomol Mass Spectrometry, Bijovet Ctr Biomed Res, NL-3584 CH Utrecht, Netherlands
[9]
Drosophila, the golden bug, emerges as a tool for human genetics
[J].
Bier, E
.
NATURE REVIEWS GENETICS,
2005, 6 (01)
:9-23

Bier, E
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Calif San Diego, Sect Cell & Dev Biol, La Jolla, CA 92039 USA Univ Calif San Diego, Sect Cell & Dev Biol, La Jolla, CA 92039 USA
[10]
Hydroponic isotope labelling of entire plants (HILEP) for quantitative plant proteomics; an oxidative stress case study
[J].
Bindschedler, Laurence V.
;
Palmblad, Magnus
;
Cramer, Rainer
.
PHYTOCHEMISTRY,
2008, 69 (10)
:1962-1972

Bindschedler, Laurence V.
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Reading, BioCtr, Reading RG6 6AS, Berks, England Univ Reading, BioCtr, Reading RG6 6AS, Berks, England

Palmblad, Magnus
论文数: 0 引用数: 0
h-index: 0
机构:
Leiden Univ, Med Ctr, Dept Parasitol, NL-2300 RC Leiden, Netherlands Univ Reading, BioCtr, Reading RG6 6AS, Berks, England

论文数: 引用数:
h-index:
机构: