Pericentromeric regions containing 1.688 satellite DNA sequences show anti-kinetochore antibody staining in prometaphase chromosomes of Drosophila melanogaster

被引：22

作者：

Abad, JP ^{[1
]}

Agudo, M ^{[1
]}

Molina, I ^{[1
]}

Losada, A ^{[1
]}

Ripoll, P ^{[1
]}

Villasante, A ^{[1
]}

机构：

[1] UAM, CSIC, Ctr Biol Mol Severo Ochoa, Madrid 28049, Spain

来源：

MOLECULAR AND GENERAL GENETICS | 2000年 / 264卷 / 04期

关键词：

satellite DNA; centromeric heterochomatin; Drosophila melanogaster;

D O I：

10.1007/s004380000331

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A striking characteristic of the centromeric heterochromatin of Drosophila melanogaster is that each chromosome carries different satellite DNA sequences. Here we show that while the major component of the 1.688 satellite DNA family expands across the centromere of the X chromosome the rest of the minor variants are located at pericentromeric positions in the large autosomes. Immunostaining of prometaphase chromosomes with the kinetocore-specific anti-BUB1 antibody reveals the transient presence of this centromeric protein in all the regions containing the 1.688 satellite.

引用

页码：371 / 377

页数：7

共 32 条

[1] DODECA SATELLITE - A CONSERVED G+C-RICH SATELLITE FROM THE CENTROMERIC HETEROCHROMATIN OF DROSOPHILA-MELANOGASTER
ABAD, JP
CARMENA, M
BAARS, S
SAUNDERS, RDC
GLOVER, DM
LUDENA, P
SENTIS, C
TYLERSMITH, C
VILLASANTE, A
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (10) : 4663 - 4667
[2] ABAD JP, 2000, IN PRESS GENETICA
[3] A dicentric chromosome of Drosophila melanogaster showing alternate centromere inactivation
Agudo, M
Abad, JP
Molina, I
Losada, A
Ripoll, P
Villasante, A
[J]. CHROMOSOMA, 2000, 109 (03) : 190 - 196
[4] Centromeres from telomeres?: The centromeric region of the Y chromosome of Drosophila melanogaster contains a tandem array of telomeric HeT-A- and TART-related sequences
Agudo, M
Losada, A
Abad, JP
Pimpinelli, S
Ripoll, P
Villasante, A
[J]. NUCLEIC ACIDS RESEARCH, 1999, 27 (16) : 3318 - 3324
[5] Mutations in the essential spindle checkpoint gene bub1 cause chromosome missegregation and fail to block apoptosis in Drosophila
Basu, J
Bousbaa, H
Logarinho, E
Li, ZX
Williams, BC
Lopes, C
Sunkel, CE
Goldberg, ML
[J]. JOURNAL OF CELL BIOLOGY, 1999, 146 (01) : 13 - 28
[6] CLONING AND CHARACTERIZATION OF A COMPLEX SATELLITE DNA FROM DROSOPHILA-MELANOGASTER
CARLSON, M
BRUTLAG, D
[J]. CELL, 1977, 11 (02) : 371 - 381
[7] DIFFERENT REGIONS OF A COMPLEX SATELLITE DNA VARY IN SIZE AND SEQUENCE OF THE REPEATING UNIT
CARLSON, M
BRUTLAG, D
[J]. JOURNAL OF MOLECULAR BIOLOGY, 1979, 135 (02) : 483 - 500
[8] CARMENA M, 1993, J CELL SCI, V105, P41
[9] DIMITRI P, 1991, GENETICS, V127, P553
[10] duSart D, 1997, NAT GENET, V16, P144

← 1 2 3 4 →