In vivo and in organello analyses of mitochondrial translation

被引：34

作者：

Fernandez-Silva, P. ^{[1
]}

Acin-Perez, R. ^{[1
]}

Fernandez-Vizarra, E. ^{[1
]}

Perez-Martos, A. ^{[1
]}

Enriquez, J. A. ^{[1
]}

机构：

[1] Univ Zaragoza, Dept Bioquim & Biol Mol & Celular, E-50013 Zaragoza, Spain

来源：

MITOCHONDRIA, 2ND EDITION | 2007年 / 80卷

关键词：

D O I：

10.1016/S0091-679X(06)80028-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Mitochondria possess their own translation system devoted to the synthesis of the mtDNA-encoded polypeptides. Analysis of the protein synthesis capacity of mitochondria can be a useful tool to study basic aspects of mtDNA expression, as well as to detect defects due to mutations in the mtDNA or in nuclear genes involved in this process. The combination of the different options for labeling and analysis of the translation products must be adapted for each particular application. © 2006 Elsevier Inc. All rights reserved.

引用

页码：571 / 588

页数：18

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