Dendritic Cells from Mycobacteria-Infected Mice Inhibits Established Allergic Airway Inflammatory Responses to Ragweed via IL-10-and IL-12-Secreting Mechanisms

被引:28
作者
Bilenki, Laura
Gao, Xiaoling
Wang, Shuhe
Yang, Jie
Fan, Yijun
Han, Xiaobing
Qiu, Hongyu
Yang, Xi [1 ]
机构
[1] Univ Manitoba, Dept Med Microbiol & Infect Dis, Fac Med, Winnipeg, MB R3E 0W3, Canada
基金
加拿大健康研究院;
关键词
ADHESION MOLECULE-1 EXPRESSION; T-CELLS; IN-VIVO; MUCUS OVERPRODUCTION; HYGIENE HYPOTHESIS; ASTHMA; MOUSE; CONTRIBUTE; BASOPHILS; IL-4;
D O I
10.4049/jimmunol.0902829
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Previous studies have demonstrated that Mycobacterium bovis bacillus Calmette-Guerin (BCG) infection can inhibit de novo and established allergen-induced asthma-like responses. The aim of this study was to examine the role of dendritic cells (DCs) in BCG infection-mediated inhibition of established allergy to a common environmental allergen-ragweed. The results showed that adoptive transfer of DCs from BCG-infected mice (DC[BCG]), in contrast to DCs from naive mice (DC[naive]), significantly inhibited established allergic airway eosinophilia and mucus overproduction. The inhibitory effect was correlated with alterations of allergen-driven cytokine and chemokine production as well as VCAM-1 expression in the lung. Flow cytometric analysis showed higher surface expression of CD8 alpha and costimulatory markers in DC(BCG) than in DC(naive). Moreover, DC(BCG) produced significantly higher levels of IL-10 and IL-12 and expressed higher levels of TLRs than did DC(naive). Furthermore, blockade of IL-10 or IL-12 significantly reversed the inhibitory effect of DC(BCG) on established allergic airway inflammation and Th2 cytokine responses. These findings suggest that DCs play a crucial role in infection-mediated inhibition of established allergic responses, and IL-10 and IL-12 production by these DCs may be a major mechanism for the inhibition. The Journal of Immunology, 2010, 184: 7288-7296.
引用
收藏
页码:7288 / 7296
页数:9
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