Differential efficacy of vaccinia virus envelope proteins administered by DNA immunisation in protection of BALB/c mice from a lethal intranasal poxvirus challenge

被引：54

作者：

Pulford, DJ

Gates, A

Bridge, SH

Robinson, JH

Ulaeto, D

机构：

[1] Biomed Sci, Salisbury SP4 0JQ, Wilts, England

[2] Univ Newcastle Upon Tyne, Fac Med Sci, Sch Clin Med Sci, Newcastle Upon Tyne NE2 4HH, Tyne & Wear, England

来源：

VACCINE | 2004年 / 22卷 / 25-26期

关键词：

DNA immunisation; B5R; gamma interferon;

D O I：

10.1016/j.vaccine.2004.02.034

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

DNA vaccines might offer an alternative to the live smallpox vaccine in providing protective efficacy in an orthopoxvirus (OPV) lethal respiratory challenge model. BALB/c mice were immunised with DNA vaccines coding for 10 different single vaccinia virus (VACV) membrane proteins. After an intranasal challenge with the VACV IHD strain, three gene candidates B5R, A33R and A27L produced >66% survival. The B5R DNA vaccine consistently produced 100% protection and exhibited greatest efficacy after three 50 mug intramuscular doses in this model. Sero-conversion to these vaccines was often inconsistent, implying that antibody itself was not a correlate of protection. The B5R DNA vaccine induced a strong and consistent gamma interferon (IFNgamma) response in BALB/c mice given a single DNA vaccine dose. Strong IFNgamma responses were also measured in pTB5R immunised C57BL6 mice deficient for MHC class I molecules, suggesting that the memory response was mediated by a CD4(+) T cell population. Crown Copyright (C) 2004 Published by Elsevier Ltd. All rights reserved.

引用

页码：3358 / 3366

页数：9

共 31 条

[11]

Fenner F., 1988, SMALLPOX ITS ERADICA

[12] USE OF A CELL-FREE SYSTEM TO IDENTIFY THE VACCINIA VIRUS L1R GENE-PRODUCT AS THE MAJOR LATE MYRISTYLATED VIRION PROTEIN M25 [J].