Poly(amino acid)-b-poly(N,N-diethylacrylamide)-b-poly(amino acid) conjugates of well-defined structure

被引：10

作者：

Bromberg, L ^{[1
]}

Levin, G ^{[1
]}

机构：

[1] Weizmann Inst Sci, Dept Mat & Interfaces, IL-76100 Rehovot, Israel

来源：

BIOCONJUGATE CHEMISTRY | 1998年 / 9卷 / 01期

关键词：

D O I：

10.1021/bc9701104

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Carboxy-terminated oligomers of N,N-diethylacrylamide (DEAAm) with low polydispersity were synthesized by group-transfer polymerization. The oligomers were conjugated with the N terminus of epsilon-N-CBZ-protected poly(lysine) and s-CBZ-protected poly(L-cysteine). The resulting conjugates precipitated in a narrow region of lower critical solution temperatures (LCST) around 29 degrees C. The LCST of poly(lysine)-polyDEAAm-poly(lysine) conjugates was pH-independent and independent of the lengths of poly(lysine) segments. Water-soluble nonstoichiometric conjugate-DNA complexes precipitated above the conjugate's LCST. Covalent complexes of human albumin and poly(cysteine)-polyDEAAm-poly(cysteine) conjugates showed temperature-sensitive behavior in aqueous solutions.

引用

页码：40 / 49

页数：10

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